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Mikrobielle Naturstoffe

Prof Dr Rolf Müller

Naturstoffe aus Mikroorganismen sind nach wie vor eine der vielversprechendsten Quellen für die Entwicklung neuer Wirkstoffe. Unsere Abteilung beschäftigt sich mit der Entdeckung, sowie der biotechnologischen Verbesserung und Produktion antimikrobieller Naturstoffe aus Myxobakterien.

Unsere Forschung

Myxobakterien sind dazu in der Lage zahlreiche Naturstoffe mit hoher struktureller und funktioneller Diversität zu produzieren. Bisher identifizierte myxobakterielle Naturstoffe zeigen Aktivitäten gegen eine Vielzahl an Organismen, von Bakterien und Pilzen bis hin zu Säugerzellen und Viren. Wir verwenden sowohl aktivitäts- als auch strukturgeleitete Ansätze um solche bioaktiven Stoffe zu identifizieren. Darüber hinaus benutzen wir auch moderne Methoden des genome mining, um das biosynthetische Potential wenig erforschter Myxobakterien zu erkunden. Im Anschluss an die Identifizierung arbeiten wir an der Aufklärung der Struktur sowie des molekularen Syntheseweges interessanter Naturstoffe. Diese Informationen schaffen die Grundlage für die biotechnologische und chemische Verbesserung der Substanzen. Unser Ziel ist es, Leitstrukturen zu ermitteln und weiter zu entwickeln, welche möglichst schnell in klinischen Studien zum Einsatz kommen sollen. Es ist uns gelungen, eine hauseigene Stammsammlung mit mehr als 10.000 einzigartigen myxobakteriellen Stämmen aufzubauen, welche aus Proben von der ganzen Welt isoliert wurden.

Team-Mitglieder

Christina Decker

Christina Decker

Sekretärin

Ellen Merckel

Ellen Merckel

Sekretärin

Yannic Nonnenmacher

Dr. Yannic Nonnenmacher

Wissenschaftlicher Referent

Marcus Miethke

Dr. Marcus Miethke

Wissenschaftlicher Referent

Carsten Volz

Dr. Carsten Volz

Labormanager / Wissenschaftler

Daniel Krug

Dr. Daniel Krug

Wissenschaftler

Jennifer Herrmann

Dr. Jennifer Herrmann

Wissenschaftlerin

Judith Hoffmann

Dr. Judith Hoffmann

Wissenschaftlerin

Ronald Garcia

Dr. Ronald Garcia

Wissenschaftler

Chantal Bader

Chantal Bader

Doktorandin

Christine Walt

Christine Walt

Doktorandin

Hu Zeng

Hu Zeng

Doktorand

Jan Schlemmer

Jan Schlemmer

Doktorand

Janetta Coetzee

Janetta Coetzee

Doktorandin

Joachim Hug

Joachim Hug

Doktorand

Joy Birkelbach

Joy Birkelbach

Doktorandin

Laura Franz

Laura Franz

Doktorandin

Markus Neuber

Markus Neuber

Doktorand

Nicolas Frank

Nicolas Frank

Doktorand

Patrick Haack

Patrick Haack

Doktorand

Sari Rasheed

Sari Rasheed

Doktorand

Yunsheng Gao

Yunsheng Gao

Doktorand

Claudia Helbig

Claudia Helbig

Technische Assistentin

Irene Kochems

Irene Kochems

Technische Assistentin

Kathrin Andres

Kathrin Andres

Technische Assistentin

Maren Andrä

Maren Andrä

Technische Assistentin

Sabine Backes

Sabine Backes

Technische Assistentin

Stefanie Schmidt

Stefanie Schmidt

Technische Assistentin

Viktoria George

Viktoria George

Technische Assistentin

Besnik Qallaku

Besnik Qallaku

Laborhelfer

Verena Qallaku

Verena Qallaku

Laborhelferin

Forschungsprojekte

Isolierung neuer Myxobakterien für die Antibiotikaforschung

Metagenomische Analysen haben gezeigt, dass in der Natur eine Vielzahl an Myxobakterien existiert, die bislang noch nicht im Labor kultiviert werden konnten. Wir entwickeln Isolierungsstrategien um genau diese Stämme in Kultur zu bringen. Unsere Suche nach Myxobakterien umfasst die Analyse verschiedener Arten von Proben, Lebensräumen, und geographischen Lagen. Ein besonderer Fokus liegt auf bislang nur wenig erforschten Gebieten (z.B. Ozeane), sowie Habitaten mit einer hohen mikrobiellen Diversität und damit einhergehendem Konkurrenzdruck (z.B. Waldböden). Myxobakterien stellen eine vielversprechende Quelle für die Entdeckung bioaktiver Naturstoffe dar und unsere bisherigen Arbeiten haben gezeigt, dass die Art produzierten Naturstoffe sich stärker unterscheidet, je höher der phylogenetische Abstand zwischen zwei Stämmen ist (d.h. je weniger eng diese miteinander verwandt sind). Dementsprechend zielt unsere Isolierungsstrategie auf bislang noch nicht kultivierte Stämme ab, welche eine hohe Chance zur Entdeckung neuartiger Antiinfektiva bieten. Die Kultivierungsbedingungen werden im Labor gezielt auf die Bedingungen im Probenmaterial angepasst um das Bakterienwachstum und die Sekundärstoffproduktion zu unterstützen. Sobald ein Stamm erfolgreich isoliert wurde, folgt eine umfangreiche physiologische Charakterisierung, eine Optimierung der Wachstumsbedingungen, sowie Fermentationen um die Stämme auf bioaktive Substanzen hin untersuchen zu können. Weiterhin wird das Genom interessanter Stämme sequenziert um aufzuklären welche Gene für die Produktion eines bestimmten Stoffes verantwortlich sind, sowie weitere, bislang unbekannte Biosynthese-Gencluster zu identifizieren. In den letzten zwei Jahren haben wir zusätzlich damit begonnen, Myxobakterien aus Bodenproben von unterschiedlichen Teilen des Saarlandes zu isolieren. Interessierte Bürgerinnen und Bürger können das HIPS bei der Probennahme im Rahmen der Mitmach-Kampagne „Sample‘ das Saarland“ unterstützen.https://www.hips.saarland/sample/

Cystobactamide als Breitbandantibiotika

Die Cystobactamide sind ein Beispiel für einen neuartigen, strukturell ungewöhnlichen Naturstoff aus Myxobakterien. Diese Gruppe nicht-ribosomal hergestellter Peptide setzt sich strukturell aus para-Aminobenzoesäure-Einheiten zusammen, welche zu einer Kette verknüpft und zusätzlich/anschließend modifiziert werden. Eine besondere Eigenschaft der Cystobactamide ist, dass sie sowohl auf grampositive als auch auf gramnegative Bakterien stark wachstumshemmend wirken. Vor allem gramnegative Erreger sind aufgrund ihrer doppelten Zellmembran üblicherweise besonders schwierig zu bekämpfen. Weiterhin zeigen die Cystobactamide keine Kreuzresistenzen mit gängigen Antibiotika wie den Fluorchinolonen. Die Kombination dieser Eigenschaften macht diese Substanzklasse zu potentiellen Kandidaten für den Einsatz als Breitbandantibiotika, weshalb sie in Kooperation mit dem Deutschen Zentrum für Infektionsforschung (DZIF) und seit Anfang 2019 auch mit der Firma Evotec weiterentwickelt werden. Das zelluläre Zielmolekül (Target) der Cystobactamide konnte mittels Analyse von Genomdaten resistenter Keime und nachfolgenden biochemischen Analysen aufgedeckt werden. Es handelt sich hierbei um die DNA-Gyrase, ein Enzym welches für die Überspiralisierung von DNA essentiell ist. Eine aus den Cystobactamiden abgeleitete Leitstruktur befindet sich mittlerweile in der präklinischen Entwicklungsphase und zeigte in Tiermodellen für bakterielle Infektionen sehr gute Aktivitäten. Damit ist der erste Schritt in der Entwicklung einer möglichen neuartigen Antibiotikaklasse getan.

Publikationen

2020

The antibiotic sorangicin A inhibits promoter DNA unwinding in a Mycobacterium tuberculosis rifampicin-resistant RNA polymerase

Lilic M, Chen J, Boyaci H, Braffman N, Hubin E, Herrmann J, Müller R, Mooney R, Landick R, Darst S, Campbell E (2020)

Proc. Natl. Acad. Sci. U.S.A.DOI: 10.1073/pnas.2013706117

Supercritical Fluid Extraction Enhances Discovery of Secondary Metabolites from Myxobacteria

Bader C, Neuber M, Panter F, Krug D, Müller R (2020)

Analytical chemistryDOI: 10.1021/acs.analchem.0c02995

An ambruticin-sensing complex modulates Myxococcus xanthus development and mediates myxobacterial interspecies communication

Marcos-Torres F, Volz C, Müller R (2020)

Nat Commun, 11 (1)DOI: 10.1038/s41467-020-19384-7

Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry

Cirnski K, Coetzee J, Herrmann J, Müller R (2020)

J. Vis. Exp. (164)DOI: 10.3791/61703

Drug Administration Routes Impact the Metabolism of a Synthetic Cannabinoid in the Zebrafish Larvae Model

Park Y, Meyer M, Müller R, Herrmann J (2020)

Molecules, 25 (19)DOI: 10.3390/molecules25194474

2-Hydroxysorangiadenosine: Structure and Biosynthesis of a Myxobacterial Sesquiterpene-Nucleoside

Okoth D, Hug J, Garcia R, Spröer C, Overmann J, Müller R (2020)

Molecules (Basel, Switzerland), 25 (11)DOI: 10.3390/molecules25112676

Biosynthesis of Cittilins, Unusual Ribosomally Synthesized and Post-translationally Modified Peptides from Myxococcus xanthus

Hug J, Dastbaz J, Adam S, Revermann O, Koehnke J, Krug D, Müller R (2020)

ACS chemical biology, 15 (8): 2221-2231DOI: 10.1021/acschembio.0c00430

Synthetic studies of cystobactamids as antibiotics and bacterial imaging carriers lead to compounds with high in vivo efficacy

Testolin G, Cirnski K, Rox K, Prochnow H, Fetz V, Grandclaudon C, Mollner T, Baiyoumy A, Ritter A, Leitner C, …, Müller R, Brönstrup M (2020)

Chemical Science, 11 (5): 1316-1334DOI: 10.1039/C9SC04769G

Bacteria as genetically programmable producers of bioactive natural products

Hug J, Krug D, Müller R (2020)

Nat. rev. chem., 4 (4): 172-193DOI: 10.1038/s41570-020-0176-1

Host Development for Heterologous Expression and Biosynthetic Studies of Myxobacterial Natural Products

Hug J, Müller R (2020)

, In pressDOI: 10.1016/B978-0-12-409547-2.14818-8

Myxobakterielle Naturstofffabriken

Krug D, Garcia R, Müller R (2020)

Biospektrum, 26 (1): 32-36DOI: 10.1007/s12268-020-1334-1

Dual-function chromogenic screening-based CRISPR/Cas9 genome editing system for actinomycetes

Wang Q, Xie F, Tong Y, Habisch R, Yang B, Zhang L, Müller R, Fu C (2020)

Applied Microbiology and Biotechnology, 104 (1): 225-239DOI: 10.1007/s00253-019-10223-4

In depth natural product discovery - Myxobacterial strains that provided multiple secondary metabolites

Bader C, Panter F, Müller R (2020)

Biotechnol Adv., 39DOI: 10.1016/j.biotechadv.2019.107480

2019

Ribosome-targeting antibiotics impair T cell effector function and ameliorate autoimmunity by blocking mitochondrial protein synthesis

Almeida L, Dhillon-LaBrooy A, Castro C, Ayele N, Bartel J, Carriche G, Guderian M, Lippens S, Dennerlein S, Hesse C, …, Moita L, Sparwasser T (2019)

Book, 97DOI: 10.1101/832956

Clinical Resistome Screening of 1,110 Escherichia coli Isolates Efficiently Recovers Diagnostically Relevant Antibiotic Resistance Biomarkers and Potential Novel Resistance Mechanisms

Volz C, Ramoni J, Beisken S, Galata V, Keller A, Plum A, Posch A, Müller R (2019)

Front. Microbiol., 10DOI: 10.3389/fmicb.2019.01671

Polyunsaturated fatty acid production by Yarrowia lipolytica employing designed myxobacterial PUFA synthases

Gemperlein K, Dietrich D, Kohlstedt M, Zipf G, Bernauer H, Wittmann C, Wenzel S, Müller R (2019)

Nat. Commun., 10 (1)DOI: 10.1038/s41467-019-12025-8

Production of a Dibrominated Aromatic Secondary Metabolite by a Planctomycete Implies Complex Interaction with a Macroalgal Host

Panter F, Garcia R, Thewes A, Zaburannyi N, Bunk B, Overmann J, Gutierrez M, Krug D, Müller R (2019)

ACS Chem. Biol., 14 (accepted // 12): 2713-2719DOI: 10.1021/acschembio.9b00641

Production optimization and biosynthesis revision of corallopyronin A, a potent anti-filarial antibiotic

Pogorevc D, Panter F, Schillinger C, Jansen R, Wenzel S, Müller R (2019)

Metab. Eng., 55: 201-211DOI: 10.1016/j.ymben.2019.07.010

Two Biosynthetic Pathways in Jahnella thaxteri for Thaxteramides, Distinct Types of Lipopeptides

Oueis E, Klefisch T, Zaburannyi N, Garcia R, Plaza A, Müller R (2019)

Org. Lett., 21 (14): 5407-5412DOI: 10.1021/acs.orglett.9b01524

The role of protein–protein interactions in the biosynthesis of ribosomally synthesized and post-translationally modified peptides

Sikandar A, Koehnke J (2019)

Nat. Prod. Rep.DOI: 10.1039/C8NP00064F

Dedication: Heinz Floss and Christopher Walsh-pioneers in natural product chemical biology

Müller R, Wright G (2019)

Journal of Industrial Microbiology & BiotechnologyDOI: 10.1007/s10295-019-02139-9

Engineering Atypical Tetracycline Formation in Amycolatopsis sulphurea for the Production of Modified Chelocardin Antibiotics

Lukežic T, Fayad A, Bader C, Harmrolfs K, Bartuli J, Groß S, Lešnik U, Hennessen F, Herrmann J, Pikl Š, Petkovic H, Müller R (2019)

ACS Chem. Biol., 14 (3): 468-477DOI: 10.1021/acschembio.8b01125

Armeniaspirol Antibiotic Biosynthesis: Chlorination and Oxidative Dechlorination Steps Affording Spiro4.4non-8-ene

Fu C, Xie F, Hoffmann J, Wang Q, Bauer A, Brönstrup M, Mahmud T, Müller R (2019)

ChemBioChem, 20 (6): 764-769DOI: 10.1002/cbic.201800791

Heterologous expression of bacterial natural product biosynthetic pathways

Huo L, Hug J, Fu C, Bian X, Zhang Y, Müller R (2019)

Nat. Prod. Rep., 36: 1412-1436DOI: 10.1039/C8NP00091C

Genome mining reveals uncommon alkylpyrones as type III PKS products from myxobacteria

Hug J, Panter F, Krug D, Müller R (2019)

J. Ind. Microbiol. Biotechnol., 46 (3-4): 319-334DOI: 10.1007/s10295-018-2105-6

Class I Methyltransferase VioH Catalyzes Unusual S-Adenosyl-l-methionine Cyclization Leading to 4-Methylazetidinecarboxylic Acid Formation during Vioprolide Biosynthesis

Yan F, Müller R (2019)

ACS Chem. Biol., 14 (1): 99-105DOI: 10.1021/acschembio.8b00958

Novel Methoxymethacrylate Natural Products Uncovered by Statistics-Based Mining of the Myxococcus fulvus Secondary Metabolome

Panter F, Krug D, Müller R (2019)

ACS Chem. Biol., 14 (1): 88-98DOI: 10.1021/acschembio.8b00948

2018

Iterative Methylations Resulting in the Biosynthesis of the t-Butyl Group Catalyzed by a B12-Dependent Radical SAM Enzyme in Cystobactamid Biosynthesis

Wang Y, Schnell B, Müller R, Begley T (2018)

Methods in enzymology, 606: 199-216DOI: 10.1016/bs.mie.2018.05.018

Adaptation of a Bacterial Multidrug Resistance System Revealed by the Structure and Function of AlbA

Sikandar A, Cirnski K, Testolin G, Volz C, Brönstrup M, Kalinina O, Müller R, Koehnke J (2018)

J. Am. Chem. Soc., 140 (48): 16641-16649DOI: 10.1021/jacs.8b08895

Characterization of an Unusual Glycerate Esterification Process in Vioprolide Biosynthesis

Auerbach D, Yan F, Zhang Y, Müller R (2018)

ACS Chem. Biol., 13 (11): 3123-3130DOI: 10.1021/acschembio.8b00826

Biocompatible bacteria-derived vesicles show inherent antimicrobial activity

Schulz E, Goes A, Garcia R, Panter F, Koch M, Müller R, Fuhrmann K, Fuhrmann G (2018)

Journal of Controlled Release, 290: 46-55DOI: 10.1016/j.jconrel.2018.09.030

A polyphasic approach leads to seven new species of the cellulose-decomposing genus Sorangium, Sorangium ambruticinum sp. nov., Sorangium arenae sp. nov., Sorangium bulgaricum sp. nov., Sorangium dawidii sp. nov., Sorangium kenyense sp. nov., Sorangium orientale sp. nov. and Sorangium reichenbachii sp. nov

Mohr K, Wolf C, Nübel U, Szafranska A, Steglich M, Hennessen F, Gemperlein K, Kämpfer P, Martin K, Müller R, Wink J (2018)

Int. J. Syst. Evol. Microbiol., 68 (11)DOI: 10.1099/ijsem.0.003034

Activation of the NLRP3 inflammasome by hyaboron, a new asymmetric boron-containing macrodiolide from the Myxobacterium Hyalangium minutum

Surup F, Chauhan D, Niggemann J, Bartok E, Herrmann J, Koeck M, Zander W, Stadler M, Hornung V, Müller R (2018)

ACS Chem. Biol., 13 (10): 2981-2988DOI: 10.1021/acschembio.8b00659

Metabolic and Biosynthetic Diversity in Marine Myxobacteria

Gemperlein K, Zaburannyi N, Garcia R, La Clair J, Müller R (2018)

Mar. Drugs, 16 (9)DOI: 10.3390/md16090314

Future Directions of Marine Myxobacterial Natural Product Discovery Inferred from Metagenomics

Garcia R, La Clair J, Müller R (2018)

Mar. Drugs, 16 (9)DOI: 10.3390/md16090303

Simulacricoccus ruber gen. nov., sp. nov., a microaerotolerant, non-fruiting, myxospore-forming soil myxobacterium and emended description of the family Myxococcaceae

Garcia R, Müller R (2018)

Int. J. Syst. Evol. Microbiol., 68 (10): 3101-3110DOI: 10.1099/ijsem.0.002936

Structure, Total Synthesis, and Biosynthesis of Chloromyxamides: Myxobacterial Tetrapeptides Featuring an Uncommon 6-Chloromethyl-5-methoxypipecolic Acid Building Block

Gorges J, Panter F, Kjaerulff L, Hoffmann T, Kazmaier U, Müller R (2018)

Angew. Chem. Int. Ed. Engl., 57 (43): 14270-14275DOI: 10.1002/anie.201808028

Concepts and Methods to Access Novel Antibiotics from Actinomycetes

Hug J, Bader C, Remškar M, Cirnski K, Müller R (2018)

Antibiotics, 7 (2)DOI: 10.3390/antibiotics7020044

Self-resistance guided genome mining uncovers new topoisomerase inhibitors from myxobacteria

Panter F, Krug D, Baumann S, Müller R (2018)

Chem. Sci., 9 (21): 4898-4908DOI: 10.1039/C8SC01325J

Biosynthesis and Heterologous Production of Vioprolides: Rational Biosynthetic Engineering and Unprecedented 4-Methylazetidinecarboxylic Acid Formation

Yan F, Auerbach D, Chai Y, Keller L, Tu Q, Hüttel S, Glemser A, Grab H, Bach T, Zhang Y, Müller R (2018)

Angew. Chem. Int. Ed. Engl., 57 (28): 8754-8759DOI: 10.1002/anie.201802479

Octapeptins: lipopeptide antibiotics against multidrug-resistant superbugs

Abou Fayad A, Herrmann J, Müller R (2018)

Cell Chem. Biol., 25 (4): 351-353DOI: 10.1016/j.chembiol.2018.04.003

Nannocystis konarekensis sp. nov., a novel myxobacterium from an Iranian desert

Mohr K, Moradi A, Glaeser S, Kämpfer P, Gemperlein K, Nübel U, Schumann P, Müller R, Wink J (2018)

Int. J. Syst. Evol. Microbiol., 68 (3): 721-729DOI: 10.1099/ijsem.0.002569

Biosynthesis of the Klebsiella oxytoca Pathogenicity Factor Tilivalline: Heterologous Expression, in Vitro Biosynthesis, and Inhibitor Development

Tesmar A, Hoffmann M, Abou Fayad A, Hüttel S, Schmitt V, Herrmann J, Müller R (2018)

ACS Chem. Biol., 13 (3): 812-819DOI: 10.1021/acschembio.7b00990

Crocadepsins-Depsipeptides from the Myxobacterium Chondromyces crocatus Found by a Genome Mining Approach

Surup F, Viehrig K, Rachid S, Plaza A, Maurer C, Hartmann R, Müller R (2018)

ACS Chem. Biol., 13 (1): 267-272DOI: 10.1021/acschembio.7b00900

Homospermidine Lipids: A compound class specifically formed during fruiting body formation of Myxococcus xanthus DK1622

Hoffmann M, Auerbach D, Panter F, Hoffmann T, Dorrestein P, Müller R (2018)

ACS Chem. Biol., 13 (1): 273-280DOI: 10.1021/acschembio.7b00816

Correlating chemical diversity with taxonomic distance for discovery of natural products in myxobacteria

Hoffmann T, Krug D, Bozkurt N, Duddela S, Jansen R, Garcia R, Gerth K, Steinmetz H, Müller R (2018)

Nat. Commun., 9 (1)DOI: 10.1038/s41467-018-03184-1

Production of myxopyronin and of its derivatives

Sucipto H, Sahner J, Wenzel S, Groh M, Hartmann R, Müller R (2018)

Patent (EP 2 994 535 B1)

2017

Novel and revisited approaches in antituberculosis drug discovery

Herrmann J, Rybniker J, Müller R (2017)

Curr. Opin. Biotechnol., 48: 94-101DOI: 10.1016/j.copbio.2017.03.023

Synthetic biology approaches to establish a heterologous production system for coronatines

Gemperlein K, Hoffmann M, Huo L, Pilak P, Petzke L, Müller R, Wenzel S (2017)

Metab. Eng., 44: 213-222DOI: 10.1016/j.ymben.2017.09.009

Heterologous production of myxobacterial α-pyrone antibiotics in Myxococcus xanthus

Sucipto H, Pogorevc D, Luxenburger E, Wenzel S, Müller R (2017)

Metab. Eng., 44: 160-170DOI: 10.1016/j.ymben.2017.10.004

Thioholgamides: Thioamide-Containing Cytotoxic RiPP Natural Products

Kjaerulff L, Sikandar A, Zaburannyi N, Adam S, Herrmann J, Koehnke J, Müller R (2017)

ACS Chem. Biol., 12 (11): 2837-2841DOI: 10.1021/acschembio.7b00676

Linoleic and palmitoleic acid block streptokinase-mediated plasminogen activation and reduce severity of invasive group A streptococcal infection

Rox K, Jansen R, Loof T, Gillen C, Bernecker S, Walker M, Chhatwal G, Müller R (2017)

Scientific Reports, 7 (1)DOI: 10.1038/s41598-017-11276-z

Total Biosynthesis of the Pyrrolo4,2benzodiazepine Scaffold Tomaymycin on an In Vitro Reconstituted NRPS System

Tesmar A, Hoffmann M, Pippel J, Fayad A, Dausend-Werner S, Bauer A, Blankenfeldt W, Müller R (2017)

Cell Chem. Biol., 24 (10): 1216-1227DOI: 10.1016/j.chembiol.2017.08.001

Biosynthesis of methyl-proline containing griselimycins, natural products with anti-tuberculosis activity

Lukat P, Katsuyama Y, Wenzel S, Binz T, König C, Blankenfeldt W, Brönstrup M, Müller R (2017)

Chem. Sci., 8 (11)DOI: 10.1039/C7SC02622F

Total syntheses of cystobactamids and structural confirmation of cystobactamid 919-2

Trauner D, Cheng B, Müller R (2017)

Angew. Chem. Int. Ed., 56 (26): 7407-7410DOI: 10.1002/anie.201705387

Pyxipyrrolones: Structure elucidation and biosynthesis of cytotoxic myxobacterial metabolites

Kjaerulff L, Raju R, Panter F, Scheid U, Garcia R, Herrmann J, Müller R (2017)

Angew. Chem. Int. Ed., 56 (32): 9614-9618DOI: 10.1002/anie.201704790

The natural product carolacton inhibits folate-dependent C1 metabolism by targeting FolD/MTHFD

Fu C, Sikandar A, Donner J, Zaburannyi N, Herrmann J, Reck M, Wagner-Döbler I, Koehnke J, Müller R (2017)

Nat Commun, 8 (1)DOI: 10.1038/s41467-017-01671-5

Vitiosangium cumulatum gen. nov., sp. nov. and Vitiosangium subalbum sp. nov., soil myxobacteria, and emended descriptions of the genera Archangium and Angiococcus, and of the family Cystobacteraceae

Awal R, Garcia R, Gemperlein K, Wink J, Kunwar B, Parajuli N, Müller R (2017)

Int. J. Syst. Evol. Microbiol., 67 (5): 1422-1430DOI: 10.1099/ijsem.0.001829

Disorazoles block group A streptococcal invasion into epithelial cells via interference with the host factor ezrin

Rox K, Rohde M, Chhatwal G, Müller R (2017)

Cell Chem. Biol., 24 (1): 159-170DOI: 10.1016/j.chembiol.2016.12.011

Structure and biosynthesis of crocagins: polycyclic postranslationally modified ribosomal peptides from Chondromyces crocatus

Viehrig K, Surup F, Volz C, Herrmann J, Abou Fayad A, Adam S, Kohnke J, Trauner D, Müller R (2017)

Angew. Chem. (56): 1-5DOI: 10.1002/anie.201612640

Solving the puzzle of the one-carbon loss in ripostatin biosynthesis

Fu C, Auerbach D, Li Y, Scheid U, Luxenburger E, Garcia R, Irschik H, Müller R (2017)

Angew. Chem. Int. Ed. Engl., 56 (8): 2192-2197DOI: 10.1002/anie.201609950

Natural products from myxobacteria: novel metabolites and bioactivities

Herrmann J, Fayad A, Müller R (2017)

Nat. Prod. Rep., 34 (2): 135-160DOI: 10.1039/C6NP00106H

2016

Racemicystis persica sp. nov., a novel myxobacterium from Iranian soil

Wink J, Moradi A, Ebrahimipour G, Mohr K, Kämpfer P, Glaeser S, Hennessen F, Gemperlein K, Awal R, Wolf C, Müller R (2016)

International Journal of Systematic and Evolutionary Microbiology, epub ahead of printDOI: 10.1099/ijsem.0.001655

Heterologous expression of the plant cysteine protease bromelain and its inhibitor in Pichia pastoris

Luniak N, Meiser P, Burkart S, Müller R (2016)

Biotechnol Progress, 33 (1): 54-65DOI: 10.1002/btpr.2405

Strategies for the discovery and development of new antibiotics from natural products: Three case studies

Herrmann J, Lukezic T, Kling A, Baumann S, Hüttel S, Petkovic H, Müller R (2016)

Contribution: 1-25DOI: 10.1007/82_2016_498

Structure and Substrate Recognition of the Bottromycin Maturation Enzyme BotP

Mann G, Huo L, Adam S, Nardone B, Vendome J, Westwood N, Muller R, Koehnke J (2016)

ChemBioChem, 17 (23): 2286-2292DOI: 10.1002/cbic.201600406

Discovery of the first small-molecule CsrA-RNA interaction inhibitors using biophysical screening technologies

Maurer C, Fruth M, Empting M, Avrutina O, Hossmann J, Nadmid S, Gorges J, Herrmann J, Kazmaier U, Dersch P, Müller R, Hartmann R (2016)

Future Med. Chem., 8 (9): 931-47DOI: 10.4155/fmc-2016-0033

Isolation, structure elucidation, and (bio)synthesis of Haprolid, a cell-type-specific myxobacterial cytotoxin

Steinmetz H, Li J, Fu C, Zaburannyi N, Kunze B, Harmrolfs K, Schmitt V, Herrmann J, Reichenbach H, Hofle G, Kalesse M, Müller R (2016)

Angew. Chem. Int. Ed. Engl., 55 (34): 10113-7DOI: 10.1002/anie.201603288

Predicting the presence of uncommon elements in unknown biomolecules from isotope patterns

Meusel M, Hufsky F, Panter F, Krug D, Müller R, Böcker S (2016)

Anal. Chem., 88: 7556-7566DOI: 10.1021/acs.analchem.6b01015

Room temperature electrocompetent bacterial cells improve DNA transformation and recombineering efficiency

Tu Q, Yin J, Fu J, Herrmann J, Li Y, Yin Y, Stewart A, Müller R, Zhang Y (2016)

Sci. Rep., 6DOI: 10.1038/srep24648

Racemicystis crocea gen. nov., sp. nov., a novel soil myxobacterium in Polyangiaceae

Awal R, Garcia R, Müller R (2016)

Int. J. Syst. Evol. Microbiol. (66)DOI: 10.1099/ijsem.0.001045

RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression

Wang H, Li Z, Jia R, Hou Y, Yin J, Bian X, Li A, Muller R, Stewart A, Fu J, Zhang Y (2016)

Nat. Protoc., 11 (7): 1175-1190DOI: 10.1038/nprot.2016.054

Genetic engineering and heterologous expression of the disorazol biosynthetic gene cluster via Red/ET recombineering

Tu Q, Herrmann J, Hu S, Raju R, Bian X, Zhang Y, Müller R (2016)

Sci. Rep., 6DOI: 10.1038/srep21066

Genome analysis of the fruiting body forming myxobacterium Chondromyces crocatus reveals high potential for natural product Biosynthesis

Zaburannyi N, Bunk B, Maier J, Overmann J, Müller R (2016)

Appl. Environ. Microbiol., 82 (6): 1945-57DOI: 10.1128/AEM.03011-15

Investigations to the antibacterial mechanism of action of kendomycin

Elnakady Y, Chatterjee I, Bischoff M, Rohde M, Josten M, Sahl H, Herrmann M, Müller R (2016)

PLoS ONE, 11 (1): 1-18DOI: 10.1371/journal.pone.0146165

Aetherobacter fasciculatus gen. nov., sp. nov. and Aetherobacter rufus gen. nov., sp. nov., two novel myxobacteria with promising biotechnological applications

Garcia R, Stadler M, Gemperlein K, Müller R (2016)

Int. J. Syst. Evol. Microbiol., 66: 928-938DOI: 10.1099/ijsem.0.000813

Metabolic engineering of Pseudomonas putida for production of docosahexaenoic acid based on a myxobacterial PUFA synthase

Gemperlein K, Zipf G, Bernauer H, Müller R, Wenzel S (2016)

Metab. Eng., 33: 98-108DOI: 10.1016/j.ymben.2015.11.001

2015

Synthetic pathway enzymes for the production of Argyrins

Müller R, Wenzel S, Garcia R (2015)

Patent (US9217169 B2)

Heterocyclyl-Butanamide Derivatives

Buchstaller H, Dorsch D (2015)

Patent (WO/2015/169421)

Two of a kind-The biosynthetic pathways of chlorotonil and anthracimycin

Jungmann K, Jansen R, Gerth K, Huch V, Krug D, Fenical W, Müller R (2015)

ACS Chem. Biol., 10 (11): 2480-2490DOI: 10.1021/acschembio.5b00523

Direct cloning and heterologous expression of the salinomycin biosynthetic gene cluster from Streptomyces albus DSM41398 in Streptomyces coelicolor A3(2)

Yin J, Hoffmann M, Bian X, Tu Q, Yan F, Xia L, Ding X, Stewart A, Müller R, Fu J, Zhang Y (2015)

Sci. Rep., 5DOI: 10.1038/srep15081

Production of the bengamide class of marine natural products in myxobacteria: biosynthesis and structure-activity relationships

Wenzel S, Hoffmann H, Zhang J, Debussche L, Haag-Richter S, Kurz M, Nardi F, Lukat P, Kochems I, Tietgen H, …, Müller R, Brönstrup M (2015)

Angew. Chem. Int. Ed. Engl., 54 (51): 15560-4DOI: 10.1002/anie.201508277

Construction of a new class of tetracycline lead structures with potent antibacterial activity through biosynthetic engineering

Lešnik U, Lukežic T, Podgoršek A, Horvat J, Polak T, Šala M, Jenko B, Harmrolfs K, Ocampo-Sosa A, Martínez-Martínez L, …, Müller R, Petkovic H (2015)

Angew. Chem. Int. Ed. Engl., 54 (13): 3937-3940DOI: 10.1002/anie.201411028

Pinensins: the first antifungal lantibiotics

Mohr K, Volz C, Jansen R, Wray V, Hoffmann J, Bernecker S, Wink J, Gerth K, Stadler M, Müller R (2015)

Angew. Chem. Int. Ed. Engl., 127 (38): 11254-11258DOI: 10.1002/anie.201500927

Cystochromones, unusual chromone-containing polyketides from the myxobacterium Cystobacter sp. MCy9104

Nadmid S, Plaza A, Garcia R, Müller R (2015)

J. Nat. Prod., 78 (8): 2023-8DOI: 10.1021/acs.jnatprod.5b00343

Macyranones: Structure, Biosynthesis, and Binding Mode of an Unprecedented Epoxyketone that Targets the 20S Proteasome

Keller L, Plaza A, Dubiella C, Groll M, Kaiser M, Müller R (2015)

J. Am. Chem. Soc., 137 (25): 8121-30DOI: 10.1021/jacs.5b03833

Biosynthetic studies of telomycin reveal new lipopeptides with enhanced activity

Fu C, Keller L, Bauer A, Brönstrup M, Froidbise A, Hammann P, Herrmann J, Mondesert G, Kurz M, Schiell M, …, Wink J, Müller R (2015)

J. Am. Chem. Soc., 137 (24): 7692-7705DOI: 10.1021/jacs.5b01794

Targeting DnaN for tuberculosis therapy using novel griselimycins

Kling A, Lukat P, Almeida D, Bauer A, Fontaine E, Sordello S, Zaburannyi N, Herrmann J, Wenzel S, König C, …, Lagrange S, Müller R (2015)

Science, 348 (6239): 1106-1112DOI: 10.1126/science.aaa4690

In Vitro reconstitution of α-Pyrone ring formation in myxopyronin biosynthesis

Sucipto H, Sahner J, Prusov E, Wenzel S, Hartmann R, Koehnke J, Müller R (2015)

Chem. Sci., 6: 5076-5085DOI: 10.1039/C5SC01013F

Two more pieces of the colibactin genotoxin puzzle from Escherichia coli show incorporation of an unusual 1-aminocyclopropanecarboxylic acid moiety

Bian X, Plaza A, Zhang Y, Müller R (2015)

Chem. Sci., 6 (5): 3154-3160DOI: 10.1039/c5sc00101c

Synthesis and biological evaluation of cystobactamid 507: a bacterial topoisomerase inhibitor from Cystobacter sp

Moreno M, Elgaher W, Herrmann J, Schläger N, Hamed M, Baumann S, Müller R, Hartmann R, Kirschning A (2015)

Synlett, 26 (9): 1175-1178DOI: 10.1055/s-0034-1380509

Rational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide derivatives in vivo

Bian X, Plaza A, Yan F, Zhang Y, Müller R (2015)

Biotechnol. Bioeng., 112 (7): 1343-53DOI: 10.1002/bit.25560

Advanced mutasynthesis studies on the natural α-pyrone antibiotic myxopyronin from Myxococcus fulvus

Sahner J, Sucipto H, Wenzel S, Groh M, Hartmann R, Müller R (2015)

ChemBioChem, 16: 946-953DOI: 10.1002/cbic.201402666

Cystodienoic acid: A new diterpene isolated from the myxobacterium Cystobacter sp.

Raju R, Mohr K, Bernecker S, Herrmann J, Müller R (2015)

J. Antibiot., 68: 473-475DOI: 10.1038/ja.2015.8

Heterologous expression of an orphan NRPS gene cluster from Paenibacillus larvae in Escherichia coli revealed production of sevadicin

Tang Y, Frewert S, Harmrolfs K, Herrmann J, Karmann L, Kazmaier U, Xia L, Zhang Y, Müller R (2015)

J. Biotechnol., 194: 112-114DOI: 10.1016/j.jbiotec.2014.12.008

Aggregicoccus edonensis gen. nov., sp. nov., an unusually aggregating myxobacterium isolated from a soil sample

Sood S, Awal R, Wink J, Mohr K, Rohde M, Stadler M, Kämpfer P, Glaeser S, Schumann P, Garcia R, Müller R (2015)

Int. J. Syst. Evol. Microbiol., 65 (Pt 3): 745-753DOI: 10.1099/ijs.0.061176-0

2014

Cystobactamids: myxobacterial topoisomerase inhibitors exhibiting potent antibacterial activity

Baumann S, Herrmann J, Raju R, Steinmetz H, Mohr K, Hüttel S, Harmrolfs K, Stadler M, Müller R (2014)

Angew. Chem. Int. Ed., 53 (52): 14605-14609DOI: 10.1002/anie.201409964

Nannozinones and Sorazinones, Unprecedented Pyrazinones from Myxobacteria

Jansen R, Sood S, Mohr K, Kunze B, Irschik H, Stadler M, Müller R (2014)

J. Nat. Prod., 77 (11): 2545-2552DOI: 10.1021/np500632c

Albaflavenol B, a new sesquiterpene isolated from the terrestrial actinomycete, Streptomyces sp.

Raju R, Gromyko O, Fedorenko V, Luzhetskyy A, Müller R (2014)

J. Antibiot., 68 (6): 286-288DOI: 10.1038/ja.2014.138

Disciformycins A and B: 12-membered macrolide glycoside antibiotics from the myxobacterium Pyxidicoccus fallax active against multiresistant staphylococci

Surup F, Viehrig K, Mohr K, Herrmann J, Jansen R, Müller R (2014)

Angew. Chem. Int. Ed. Engl., 49 (53): 13588-13591DOI: 10.1002/anie.201406973

Improving natural products identification through targeted LC-MS/MS in an untargeted secondary metabolomics workflow

Hoffmann T, Krug D, Hüttel S, Müller R (2014)

Anal. Chem., 86 (21): 10780-10788DOI: 10.1021/ac502805w

Minicystis rosea gen. nov., sp. nov., a polyunsaturated fatty acid-rich and steroid-producing soil myxobacterium

Garcia R, Gemperlein K, Müller R (2014)

Int. J. Syst. Evol. Microbiol., 64 (Pt 11): 3733-3742DOI: 10.1099/ijs.0.068270-0

Cystobactamid und Derivate als Antibiotika

Baumann S, Herrmann J, Mohr K, Steinmetz H, Gerth K, Raju R, Müller R (2014)

Patent (1451)

Paenilarvins: Iturin family lipopeptides from the honey bee pathogen Paenibacillus larvae

Sood S, Steinmetz H, Beims H, Mohr K, Stadler M, Djukic M, Ohe W, Steinert M, Daniel R, Müller R (2014)

ChemBioChem, 15 (13): 1947-1955DOI: 10.1002/cbic.201402139

Antimalarial activity of the myxobacterial macrolide chlorotonil A

Held J, Gebru T, Kalesse M, Jansen R, Gerth K, Müller R, Mordmüller B (2014)

Antimicrob. Agents Chemother., 58 (11): 6378-6384DOI: 10.1128/AAC.03326-14

Heterologous production of glidobactins/luminmycins in Escherichia coli nissle containing the glidobactin biosynthetic gene cluster from Burkholderia DSM7029

Bian X, Huang F, Wang H, Klefisch T, Müller R, Zhang Y (2014)

ChemBioChem, 15 (15): 2221-2224DOI: 10.1002/cbic.201402199

A highly unusual polyketide synthase directs dawenol polyene biosynthesis in Stigmatella aurantiaca

Oßwald C, Zaburannyi N, Burgard C, Hoffmann T, Wenzel S, Müller R (2014)

J. Biotechnol., 191: 54-63DOI: 10.1016/j.jbiotec.2014.07.447

Targeting the actin cytoskeleton: selective anti-tumor action via trapping PKCå

Vollmar A, Förster F, Braig S, Moser C, Kubisch R, Busse J, Wagner E, Schmöckel E, Mayr D, Schmitt S, …, Zischka H, Müller R (2014)

Cell Death & Disease, 5DOI: 10.1038/cddis.2014.363

Hyalachelins A-C, unusual siderophores isolated from the terrestrial myxobacterium Hyalangium minutum

Nadmid S, Plaza A, Lauro G, Garcia R, Bifulco G, Müller R (2014)

Org. Lett., 16 (16): 4130-4133DOI: 10.1021/ol501826a

Hyafurones, hyapyrrolines, and hyapyrones: polyketides from Hyalangium minutum

Okanya P, Mohr K, Gerth K, Kessler W, Jansen R, Stadler M, Müller R (2014)

J. Nat. Prod., 77 (6): 1420-1429DOI: 10.1021/np500145f

Biosynthesis of crocacin involves an unusual hydrolytic release domain showing similarity to condensation domains

Müller S, Rachid S, Hoffmann T, Surup F, Volz C, Zaburannyi N, Müller R (2014)

Chem. Biol., 21 (7): 855-865DOI: 10.1016/j.chembiol.2014.05.012

Angiolactone, a new Butyrolactone isolated from the terrestrial myxobacterium, Angiococcus sp.

Raju R, Garcia R, Müller R (2014)

J. Antibiot., epub ahead of printDOI: 10.1038/ja.2014.55

Cystomanamides: structure and biosynthetic pathway of a family of glycosylated lipopeptides from myxobacteria

Etzbach L, Plaza A, Garcia R, Baumann S, Müller R (2014)

Org. Lett., 16 (9): 2414-2417DOI: 10.1021/ol500779s

Indothiazinone, an indolyl-thiazolyl-ketone from a novel myxobacterium belonging to the Sorangiineae

Jansen R, Mohr K, Bernecker S, Stadler M, Müller R (2014)

J. Nat. Prod., 77 (4): 1054-1060DOI: 10.1021/np500144t

Secondary metabolomics: the impact of mass spectrometry-based approaches on the discovery and characterization of microbial natural products

Krug D, Müller R (2014)

Nat. Prod. Rep., 31 (6): 768-783DOI: 10.1039/c3np70127a

Polyunsaturated fatty acid biosynthesis in myxobacteria

Gemperlein K, Rachid S, Garcia R, Wenzel S, Müller R (2014)

Chem. Sci., 5 (5): 1733-1741DOI: 10.1039/C3SC53163E

Biosynthesis of magnetic nanostructures in a foreign organism by transfer of bacterial magnetosome gene clusters

Kolinko I, Lohße A, Borg S, Raschdorf R, Jogler C, Tu Q, Pósfai M, Tompa E, Plitzko J, Brachmann A, …, Zhang Y, Schüler D (2014)

Nat. Nanotechnol., 9 (3): 193-197DOI: 10.1038/nnano.2014.13

Pyrronazols, metabolites from the myxobacteria Nannocystis pusilla and N. exedens, are unusual chlorinated pyrone-oxazole-pyrroles

Jansen R, Sood S, Huch V, Kunze B, Stadler M, Müller R (2014)

J. Nat. Prod., 77 (2): 320-326DOI: 10.1021/np400877r

Oleamycins A and B: new antibacterial cyclic hexadepsipeptides isolated from a terrestrial Streptomyces sp.

Raju R, Gromyko O, Andriy B, Fedorenko V, Luzhetskyy A, Müller R (2014)

Journal of Antibiotics (Tokyo), 67 (4): 339-343DOI: 10.1038/ja.2014.1

Improved seamless mutagenesis by recombineering using ccdB for counterselection

Wang H, Bian X, Xia L, Ding X, Müller R, Zhang Y, Fu J, Stewart A (2014)

Nucleic Acids Res., 42 (5)DOI: 10.1093/nar/gkt1339

Modular Construction of a Functional Artificial Epothilone Polyketide Pathway

Oßwald C, Zipf G, Schmidt G, Maier J, Bernauer H, Müller R, Wenzel S (2014)

ACS Synth. Biol., 3 (10): 759-772DOI: 10.1021/sb300080t

Future potential for anti-infectives from bacteria - How to exploit biodiversity and genomic potential

Müller R, Wink J (2014)

Int. J. Med. Microbiol., 304 (1): 3-13DOI: 10.1016/j.ijmm.2013.09.004

2013

Novel macrolide antibiotics

Müller R, Nett M, Surup F, Steinmetz H, Mohr K, Viehrig K (2013)

Patent

Jahnellamides, a-keto-ß-methionine-containing peptides from the terrestrial myxobacterium Jahnella sp.: structure and biosynthesis

Plaza A, Viehrig K, Garcia R, Müller R (2013)

Org. Lett., 15 (22): 5882-5885DOI: 10.1021/ol402967y

Microsclerodermins from terrestrial myxobacteria: An intriguing biosynthesis likely connected to a sponge symbiont

Hoffmann T, Müller S, Nadmid S, Garcia R, Müller R (2013)

J. Am. Chem. Soc., 45 (135): 16904-16911DOI: 10.1021/ja4054509

Analytics of the therapeutic peptide aviptadil by sheathless CE-MS and comparison with nanoRP-HPLC-MS

Groß P, Burkart S, Müller R (2013)

J. Pharm. Biomed. Anal., 88 (2014): 477-482DOI: 10.1016/j.jpba.2013.09.024

Concerted action of P450 plus helper protein to form the amino-hydroxy-piperidone moiety of the potent protease inhibitor crocapeptin

Viehrig K, Surup F, Harmrolfs K, Jansen R, Kunze B, Müller R (2013)

J. Am. Chem. Soc., 135 (45): 16885-16894DOI: 10.1021/ja4047153

Production of fatty acids by heterologous expression of gene clusters from myxobacteria

Wenzel S, Rachid S, Gemperlein K, Müller R (2013)

Patent, AC12P764FI (WO 2011/151298 A1)

Discovery and biological activity of new chondramides from Chondromyces sp.

Herrmann J, Hüttel S, Müller R (2013)

ChemBioChem, 14 (13): 1573-1580DOI: 10.1002/cbic.201300140

Synthesis and biological activities of the respiratory chain inhibitor aurachin D and new ring versus chain analogues

Li X, Herrmann J, Zang Y, Grellier P, Prado S, Müller R, Nay B (2013)

Beilstein J. Org. Chem., 9: 1551-1558DOI: 10.3762/bjoc.9.176

Oleaceran: A novel spiro[isobenzofuran-1,2'-naptho[1,8-bc]furan] isolated from a terrestrial Streptomyces sp

Raju R, Gromyko O, Fedorenko V, Luzhetskyy A, Müller R (2013)

Org. Lett., 15 (14): 3487-3489DOI: 10.1021/ol401490u

Recent advances in the heterologous expression of microbial natural product biosynthetic pathways

Ongley S, Bian X, Neilan B, Müller R (2013)

Nat. Prod. Rep., 30 (8): 1121-1138DOI: 10.1039/c3np70034h

In vivo evidence for a prodrug activation mechanism during colibactin maturation

Bian X, Fu J, Plaza A, Herrmann J, Pistorius D, Stewart A, Zhang Y, Müller R (2013)

ChemBioChem, 14 (10): 1194-1197DOI: 10.1002/cbic.201300208

Lorneic acids C and D, new trialkyl-substituted aromatic acids isolated from a terrestrial Streptomyces sp.

Raju R, Gromyko O, Fedorenko V, Luzhetskyy A, Müller R (2013)

J. Antibiot., 66 (6): 347-349DOI: 10.1038/ja.2013.18

Isolation, structure elucidation, and biological activity of maltepolides: remarkable macrolides from myxobacteria

Irschik H, Washausen P, Sasse F, Fohrer J, Huch V, Müller R, Prusov E (2013)

Angew. Chem. Int. Ed. Engl., 52 (20): 5402-5405DOI: 10.1002/anie.201210113

Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature

Arnison P, Bibb M, Bierbaum G, Bowers A, Bugni T, Bulaj G, Camarero J, Campopiano D, Challis G, Clardy J, …, Willey J, van der Donk W (2013)

Nat. Prod. Rep., 30 (1): 108-160DOI: 10.1039/C2NP20085F

Activity-guided screening of bioactive natural compounds implementing a new glucocorticoid-receptor-translocation assay and detection of new anti-inflammatory steroids from bacteria

Kaufmann K, Simmons L, Herrmann J, Schwär G, Luniak N, Müller R (2013)

Biotechnol. Lett., 35 (1): 11-20DOI: 10.1007/s10529-012-1042-0

2012

Method for producing recombinant 11-de-O-methyltomaymycin

Lattemann C, Broenstrup M, Werner S, Müller R, Harmrolfs K (2012)

Patent, 2012 (EP2849753)

Pellasoren: structure elucidation, biosynthesis, and total synthesis of a cytotoxic secondary metabolite from Sorangium cellulosum

Jahns C, Hoffmann T, Müller S, Gerth K, Washausen P, Höfle G, Reichenbach H, Kalesse M, Müller R (2012)

Angew. Chem. Int. Ed. Engl., 51 (21): 5239-5243DOI: 10.1002/anie.201200327

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting

Fu J, Bian X, Hu S, Wang H, Huang F, Seibert P, Plaza A, Xia L, Müller R, Stewart A, Zhang Y (2012)

Nat. Biotechnol., 30 (5): 440-446DOI: 10.1038/nbt.2183

Rubimycinone A, a new anthraquinone from a terrestrial Streptomyces sp.

Raju R, Gromyko O, Fedorenko V, Herrmann J, Luzhetskyy A, Müller R (2012)

Tetrahedron Lett., 54 (8): 900-902DOI: 10.1016/j.tetlet.2012.11.130

Juniperolide A: a new polyketide isolated from a terrestrial actinomycete, Streptomyces sp.

Raju R, Gromyko O, Fedorenko V, Luzhetskyy A, Plaza A, Müller R (2012)

Org. Lett., 14 (23): 5860-5863DOI: 10.1021/ol302766z

Ein alternativer isovaleryl-CoA-biosyntheseweg: beteiligung einer bisher unbekannten 3-methylglutaconyl-CoA-decarboxylase

Li Y, Luxenburger E, Müller R (2012)

Angew. Chem.DOI: 10.1002/ange.201207984

An alternative isovaleryl CoA biosynthetic pathway involving a previously unknown 3-methylglutaconyl CoA decarboxylase

Li Y, Luxenburger E, Müller R (2012)

Angew. Chem. Int. Ed. Engl., 52 (4): 1304-1308DOI: 10.1002/anie.201207984

Synthetic biotechnology to study and engineer ribosomal bottromycin biosynthesis

Huo L, Rachid S, Stadler M, Wenzel S, Müller R (2012)

Chem. Biol., 19 (10): 1278-1287DOI: 10.1016/j.chembiol.2012.08.013

Enhancer binding proteins act as hetero-oligomers and link secondary metabolite production to myxococcal development, motility and predation

Volz C, Kegler C, Müller R (2012)

Chem. Biol., 19: 1447-1459DOI: 10.1016/j.chembiol.2012.09.010

Indiacens A and B: prenyl indoles from the myxobacterium Sandaracinus amylolyticus

Steinmetz H, Mohr K, Zander W, Jansen R, Gerth K, Müller R (2012)

J. Nat. Prod., 75 (10): 1803-1805DOI: 10.1021/np300288b

Leopolic acid A, isolated from a terrestrial actinomycete, Streptomyces sp.

Raju R, Gromyko O, Fedorenko V, Luzhetskyy A, Müller R (2012)

Tetrahedron Lett., 53 (46): 6300-6301DOI: 10.1016/j.tetlet.2012.09.046

Aetheramides A and B, potent HIV-inhibitory depsipeptides from a myxobacterium of the new genus 'Aetherobacter'

Plaza A, Garcia R, Bifulco G, Martinez J, Hüttel S, Sasse F, Meyerhans A, Stadler M, Müller R (2012)

Org. Lett., 14 (11): 2854-2857DOI: 10.1021/ol3011002

Precursor-directed syntheses and biological evaluation of new elansolid derivatives

Steinmetz H, Zander W, Shushni M, Jansen G, Dehn R, Dräger G, Kirschning A, Müller R (2012)

ChemBioChem, 13 (12): 1813-1817DOI: 10.1002/cbic.201200228

Pretubulysin: From hypothetical biosynthetic intermediate to potential lead in tumor therapy

Herrmann J, Elnakady Y, Wiedmann R, Ullrich A, Rohde M, Kazmaier U, Vollmar A, Müller R (2012)

PLoS ONE, 7 (5)DOI: 10.1371/journal.pone.0037416

Luminmycins A-C, cryptic natural products from Photorhabdus luminescens identified by heterologous expression in Escherichia coli

Bian X, Plaza A, Zhang Y, Müller R (2012)

J. Nat. Prod., 75 (9): 1652-1655DOI: 10.1021/np300444e

Methods to optimize myxobacterial fermentations using off-gas analysis

Hüttel S, Müller R (2012)

Microb. Cell Fact., 11 (59)DOI: 10.1186/1475-2859-11-59

Eine Semipinakol-Umlagerung - katalysiert durch ein Enzymsystem mit difunktioneller FAD-abhängiger Monooxygenase

Katsuyama Y, Harmrolfs K, Pistorius D, Li Y, Müller R (2012)

Angew. Chem. Int. Ed. Engl., 124 (37): 9573-9576DOI: 10.1002/ange.201204138

Direct cloning, genetic engineering, and heterologous expression of the syringolin biosynthetic gene cluster in E. coli through Red/ET recombineering

Bian X, Huang F, Stewart F, Xia L, Zhang Y, Müller R (2012)

ChemBioChem, 13 (13): 1946-1952DOI: 10.1002/cbic.201200310

A semipinacol rearrangement directed by an enzymatic system featuring dual-function FAD-dependent monooxygenase

Katsuyama Y, Harmrolfs K, Pistorius D, Li Y, Müller R (2012)

Angew. Chem. Int. Ed. Engl., 51 (37): 9437-9440DOI: 10.1002/anie.201204138

Myxobacterium-produced antibiotic TA (myxovirescin) inhibits type II signal peptidase

Xiao Y, Gerth K, Müller R, Wall D (2012)

Antimicrob. Agents Chemother., 56 (4): 2014-2021DOI: 10.1128/AAC.06148-11

Pimprinols A-C, from the terrestrial actinomycete, Streptomyces sp.

Raju R, Gromyko O, Fedorenko V, Luzhetskyy A, Müller R (2012)

Tetrahedron Lett., 53 (24): 3009-3011DOI: 10.1016/j.tetlet.2012.03.134

Hyaladione, an S-methyl cyclohexadiene-dione from Hyalangium minutum

Okanya P, Mohr K, Gerth K, Steinmetz H, Huch V, Jansen R, Müller R (2012)

J. Nat. Prod., 75 (4): 768-770DOI: 10.1021/np200776v

Heterologous expression and genetic engineering of the tubulysin biosynthetic gene cluster using Red/ET recombineering and inactivation mutagenesis

Chai Y, Shan S, Weissman K, Hu S, Zhang Y, Müller R (2012)

Chem. Biol., 19 (3): 361-371DOI: 10.1016/j.chembiol.2012.01.007

Isolation and total synthesis of icumazoles and noricumazoles—antifungal antibiotics and cation-channel blockers from Sorangium cellulosum

Barbier J, Jansen R, Irschik H, Benson S, Gerth K, Böhlendorf B, Höfle G, Reichenbach H, Wegner J, Zeilinger C, Kirschning A, Müller R (2012)

Angew. Chem. Int. Ed. Engl., 51 (5): 1256-1260DOI: 10.1002/anie.201106435

Myxoprincomide: a natural product from Myxococcus xanthus discovered by comprehensive analysis of the secondary metabolome

Cortina N, Krug D, Plaza A, Revermann O, Müller R (2012)

Angew. Chem. Int. Ed. Engl., 51 (3): 811-816DOI: 10.1002/anie.201106305

Unusual carbon fixation gives rise to diverse polyketide extender units

Quade N, Huo L, Rachid S, Heinz D, Müller R (2012)

Nat. Chem. Biol., 8 (1): 117-124DOI: 10.1038/nchembio.734

Sandaracinus amylolyticus gen. nov., sp. nov., a starch-degrading soil myxobacterium, and description of Sandaracinaceae fam. nov

Mohr K, Garcia R, Gerth K, Irschik H, Müller R (2012)

Int. J. Syst. Evol. Microbiol., 62 (Pt 5): 1191-1198DOI: 10.1099/ijs.0.033696-0

Discovery of the rhizopodin biosynthetic gene cluster in Stigmatella aurantiaca Sg a15 by genome mining

Pistorius D, Müller R (2012)

Chembiochem : a European journal of chemical biology, 13 (3): 416-426DOI: 10.1002/cbic.201100575

2011

Completing the puzzle of aurachin biosynthesis in Stigmatella aurantiaca Sg a15

Pistorius D, Li Y, Sandmann A, Müller R (2011)

Mol. Biosyst., 7 (12): 3308-3315DOI: 10.1039/c1mb05328k

AuaA, a membrane-bound farnesyltransferase from Stigmatella aurantiaca, catalyzes the prenylation of 2-methyl-4-hydroxyquinoline in the biosynthesis of aurachins

Stec E, Pistorius D, Müller R, Li S (2011)

ChemBioChem, 12 (11): 1724-1730DOI: 10.1002/cbic.201100188

Unprecedented anthranilate priming involving two enzymes of the acyl adenylating superfamily in aurachin biosynthesis

Pistorius D, Li Y, Mann S, Müller R (2011)

J. Am. Chem. Soc., 133 (32): 12362-12365DOI: 10.1021/ja203653w

A highly conjugated dihydroxylated C28 steroid from a myxobacterium

Gawas D, Garcia R, Huch V, Müller R (2011)

Journal of natural products, 74 (5): 1281-1283DOI: 10.1021/np100682c

Bendigoles D-F, bioactive sterols from the marine sponge-derived Actinomadura sp. SBMs009

Simmons L, Kaufmann K, Garcia R, Schwär G, Huch V, Müller R (2011)

Bioorganic & Medicinal Chemistry, 19 (22): 6570-6575DOI: 10.1016/j.bmc.2011.05.044

Elansolid A3, a unique p-quinone methide antibiotic from Chitinophaga sancti

Jansen R, Gerth K, Steinmetz H, Reinecke S, Kessler W, Kirschning A, Müller R (2011)

Chem. Eur. J., 17 (28): 7739-7744DOI: 10.1002/chem.201100457

Molecular basis of elansolid biosynthesis: evidence for an unprecedented quinone methide initiated intramolecular Diels-Alder cycloaddition/macrolactonization

Dehn R, Katsuyama Y, Weber A, Gerth K, Jansen R, Steinmetz H, Höfle G, Müller R, Kirschning A (2011)

Angew. Chem. Int. Ed. Engl., 50 (17): 3882-3887DOI: 10.1002/anie.201006880

Roimatacene: an antibiotic against gram-negative bacteria isolated from Cystobacter ferrugineus Cb G35 (myxobacteria)

Zander W, Gerth K, Mohr K, Kessler W, Jansen R, Müller R (2011)

Chem. Eur. J., 17 (28): 7875-7881DOI: 10.1002/chem.201003677

p-Hydroxyacetophenone amides from Cystobacter ferrugineus, strain Cb G35

Zander W, Mohr K, Gerth K, Jansen R, Müller R (2011)

J. Nat. Prod., 74 (6): 1358-1363DOI: 10.1021/np1006789

Identification and characterization of the althiomycin biosynthetic gene cluster in Myxococcus xanthus DK897

Cortina N, Revermann O, Krug D, Müller R (2011)

ChemBioChem, 12 (9): 1411-1416DOI: 10.1002/cbic.201100154

Fatty acid-related phylogeny of myxobacteria as an approach to discover polyunsaturated omega-3/6 fatty acids

Garcia R, Pistorius D, Stadler M, Müller R (2011)

J. Bacteriol., 193 (8): 1930-1942DOI: 10.1128/JB.01091-10

Biosynthesis of 2-alkyl-4(1H)-quinolones in Pseudomonas aeruginosa: potential for therapeutic interference with pathogenicity

Pistorius D, Ullrich A, Lucas S, Hartmann R, Kazmaier U, Müller R (2011)

ChemBioChem, 12 (6): 850-853DOI: 10.1002/cbic.201100014

Marinoquinolines A-F, Pyrroloquinolines from Ohtaekwangia kribbensis (Bacteroidetes)

Okanya P, Mohr K, Gerth K, Jansen R, Müller R (2011)

J. Nat. Prod., 74 (4): 603-608DOI: 10.1021/np100625a

Insights into the complex biosynthesis of the leupyrrins in Sorangium cellulosum So ce690

Kopp M, Irschik H, Gemperlein K, Buntin K, Meiser P, Weissman K, Bode H, Müller R (2011)

Mol. Biosyst., 7 (5): 1549-1563DOI: 10.1039/c0mb00240b

Mining the cinnabaramide biosynthetic pathway to generate novel proteasome inhibitors

Rachid S, Huo L, Herrmann J, Stadler M, Köpcke B, Bitzer J, Müller R (2011)

ChemBioChem, 12 (6): 922-931DOI: 10.1002/cbic.201100024

Elansolid A, a unique macrolide antibiotic from Chitinophaga sancti isolated as two stable atropisomers

Steinmetz H, Gerth K, Jansen R, Schläger N, Dehn R, Reinecke S, Kirschning A, Müller R (2011)

Angew. Chem. Int. Ed. Engl., 50 (2): 532-536DOI: 10.1002/anie.201005226

2010

Heterologous hosts

Zhang Y, Fu J, Bian X, Stewart A, Müller R (2010)

Patent (US 9,580,717 B2)

The CYPome of Sorangium cellulosum So ce56 and identification of CYP109D1 as a new fatty acid hydroxylase

Khatri Y, Hannemann F, Ewen K, Pistorius D, Perlova O, Kagawa N, Brachmann A, Müller R, Bernhardt R (2010)

Chem. Biol., 17: 1295-1305DOI: 10.1016/j.chembiol.2010.10.010

Production of omega-3 fatty acids by myxobacteria International Patent WO 2010/063451 A2

Stadler M, Roemer E, Müller R, Garcia R, Pistorius D, Brachmann A (2010)

Patent (International Patent WO 2010/063451 A2)

Volatile Methyl Esters of Medium Chain Length from the Bacterium Chitinophaga Fx7914

Nawrath T, Gerth K, Müller R, Schulz S (2010)

Chemistry & Biodiversity, 7 (9): 2228-2253

The Biosynthesis of the Aroma Volatile 2-Methyltetrahydrothiophen-3-one in the Bacterium Chitinophaga Fx7914

Nawrath T, Gerth K, Müller R, Schulz S (2010)

ChemBioChem, 11 (13): 1914-1919DOI: 10.1002/cbic.201000296

Expanded phylogeny of myxobacteria and evidence for cultivation of the 'unculturables'

Garcia R, Gerth K, Stadler M, Dogma Jr. I, Müller R (2010)

Mol. Phylogenet. Evol., 57 (2): 878-887DOI: 10.1016/j.ympev.2010.08.028

Insights into an unusual nonribosomal peptide synthetase biosynthesis: identification and characterization of the GE81112 biosynthetic gene cluster

Binz T, Maffioli S, Sosio M, Donadio S, Müller R (2010)

J. Biol. Chem., 285 (43): 32710-32719DOI: 10.1074/jbc.M110.146803

Analysis of the sorangicin gene cluster reinforces the utility of a combined phylogenetic/retrobiosynthetic analysis for deciphering natural product assembly by trans-AT PKS

Irschik H, Kopp M, Weissman K, Buntin K, Piel J, Müller R (2010)

ChemBioChem, 11 (13): 1840-1849DOI: 10.1002/cbic.201000313

Functions of genes and enzymes involved in phenalinolactone biosynthesis

Daum M, Schnell H, Herrmann S, Günther A, Murillo R, Müller R, Bisel P, Müller M, Bechtlold A (2010)

ChemBioChem, 11 (10): 1383-1391DOI: 10.1002/cbic.201000117

Myxobacterial secondary metabolites: bioactivities and modes-of-action

Weissman K, Müller R (2010)

Nat. Prod. Rep., 27 (9): 1276-1295DOI: 10.1039/c001260m

Biosynthesis of thuggacins in myxobacteria: comparative cluster analysis reveals basis for natural product structural diversity

Buntin K, Irschik H, Weissman K, Luxenburger E, Blöcker H, Müller R (2010)

Chem. Biol., 17 (4): 342-356DOI: 10.1016/j.chembiol.2010.02.013

Biosynthesis of the myxobacterial antibiotic corallopyronin A

Erol Ö, Schäberle T, Schmitz A, Rachid S, Gurgui C, El Omari M, Lohr F, Kehraus S, Piel J, Müller R, König G (2010)

ChemBioChem, 11 (9): 1235-1265DOI: 10.1002/cbic.201000085

Carolacton - a Macrolide Ketocarbonic Acid Reducing Biofilm Formation by the Caries- and Endocarditis-associated Bacterium Streptococcus mutans

Jansen R, Irschik H, Huch V, Schummer D, Steinmetz H, Bock M, Schmidt T, Kirschning A, Müller R (2010)

Eur. J. Org. Chem., 2010 (7): 1284-1289DOI: 10.1002/ejoc.200901126

An unusual thioesterase promotes isochromanone ring formation in ajudazol biosynthesis

Buntin K, Weissman K, Müller R (2010)

ChemBioChem, 11 (8): 1137-1146DOI: 10.1002/cbic.200900712

Insights into multienzyme docking in hybrid PKS-NRPS megasynthetases revealed by heterologous expression and genetic engineering

Li Y, Weissman K, Müller R (2010)

ChemBioChem, 11 (8): 1069-1075DOI: 10.1002/cbic.201000103

Discovery of 23 natural tubulysins from Angiococcus disciformis An d48 and Cystobacter SBCb004

Chai Y, Pistorius D, Ullrich A, Weissman K, Kazmaier U, Müller R (2010)

Chem. Biol., 17 (3): 296-309DOI: 10.1016/j.chembiol.2010.01.016

Characterization of a novel type of oxidative decarboxylase involved in the biosynthesis of the styryl moiety of chondrochloren from an acylated tyrosine

Rachid S, Revermann O, Dauth C, Müller R (2010)

J. Biol. Chem., 285 (17): 12482-12489DOI: 10.1074/jbc.M109.079707

2009

Pretubulysin, a potent and chemically accessible tubulysin precursor from Angiococcus disciformis

Ullrich A, Chai Y, Pistorius D, Elnakady Y, Herrmann J, Weissman K, Kazmaier U, Müller R (2009)

Angew. Chem. Int. Ed. Engl., 48 (24): 4422-4425DOI: 10.1002/anie.200900406

Progress in the chemistry of organic natural products

Altmann K, Höfle G, Müller R, Mulzer J, Prantz K (2009)

CollectedWorks

Synthesis and Biological Evaluation of Pretubulysin and Derivatives

Ullrich A, Herrmann J, Müller R, Kazmaier U (2009)

Eur. J. Org. Chem., 2009 (36): 6367-6378

Genome mining in Sorangium cellulosum So ce56 - identification and characterization of the homologous electron transfer proteins of a myxobacterial cytochrome P450

Ewen K, Hannemann F, Khatri Y, Perlova O, Kappl R, Krug D, Hüttermann J, Müller R, Bernhardt R (2009)

J. Biol. Chem., 284 (42): 28590-28598DOI: 10.1074/jbc.M109.021717

The impact of genomics on the exploitation of the myxobacterial secondary metabolome

Wenzel S, Müller R (2009)

Nat. Prod. Rep., 26 (11): 1385-1407DOI: 10.1039/b817073h

Non-modular polyketide synthases in myxobacteria

Li Y, Müller R (2009)

Phytochemistry, 70 (15-16): 1850-1857

Myxobacteria--'microbial factories' for the production of bioactive secondary metabolites

Wenzel S, Müller R (2009)

Mol. Biosyst., 5 (6): 567-574DOI: 10.1039/b901287g

The biosynthetic potential of myxobacteria and their impact on drug discovery

Wenzel S, Müller R (2009)

Curr. Opin. Drug Discov. Devel., 12 (2): 220-230

Structure and action of the myxobacterial chondrochloren halogenase CndH: a new variant of FAD-dependent halogenases

Buedenbender S, Rachid S, Müller R, Schulz G (2009)

J. Mol. Biol., 385 (2): 520-530DOI: 10.1016/j.jmb.2008.10.057

Novel expression hosts for complex secondary metabolite megasynthetases: Production of myxochromide in the thermopilic isolate Corallococcus macrosporus GT-2

Perlova O, Gerth K, Kuhlmann S, Zhang Y, Müller R (2009)

Microb. Cell Fact., 8 (1)DOI: 10.1186/1475-2859-8-1

Identification of additional players in the alternative biosynthesis pathway to isovaleryl-CoA in the myxobacterium Myxococcus xanthus

Bode H, Ring M, Schwär G, Altmeyer M, Kegler C, Jose I, Singer M, Müller R (2009)

ChemBioChem, 10 (1): 128-140DOI: 10.1002/cbic.200800219

Unusual chemistry in the biosynthesis of the antibiotic chondrochlorens

Rachid S, Scharfe M, Blöcker H, Weissman K, Müller R (2009)

Chem. Biol., 16 (1): 70-81DOI: 10.1016/j.chembiol.2008.11.005

Discovery of additional members of the tyrosine aminomutase enzyme family and the mutational analysis of CmdF

Krug D, Müller R (2009)

ChemBioChem, 10 (4): 741-750DOI: 10.1002/cbic.200800748

Phaselicystis flava gen. nov., sp. nov., an arachidonic acid-containing soil myxobacterium, and the description of Phaselicystidaceae fam. nov

Garcia R, Reichenbach H, Ring M, Müller R (2009)

Int. J. Syst. Evol. Microbiol., 59 (6): 1524-1530DOI: 10.1099/ijs.0.003814-0

A brief tour of myxobacterial secondary metabolism

Weissman K, Müller R (2009)

Bioorganic & Medicinal Chemistry, 17 (6): 2121-2136DOI: 10.1016/j.bmc.2008.11.025

2008

Epothilones - a fascinating family of microtubule stabilizing antitumor agents

Mulzer J, Altmann K, Hofle G, Müller R, Prantz K (2008)

Comptes Rendus Chimie, 11 (11-12): 1336-1368DOI: 10.1016/j.crci.2008.02.005

Isolation and structure revision of the actin-binding macrolide rhizopodin from Myxococcus stipitatus (Myxobacteria)

Jansen R, Steinmetz H, Sasse F, Schubert W, Hagelueken G, Albrecht S, Müller R (2008)

Tetrahedron Lett., 49 (40): 5796-5799DOI: 10.1016/j.tetlet.2008.07.132

NtcA-A negative regulator of secondary metabolite biosynthesis in Sorangium cellulosum

Rachid S, Gerth K, Müller R (2008)

J. Biotechnol., 140 (1-2): 135-142DOI: 10.1016/j.jbiotec.2008.10.010

Epothilones - A fascinating family of microtubule stabilizing antitumor agents

Mulzer J, Altmann K, Höfle G, Müller R, Prantz K (2008)

C. R. Chimie, 11

A type I/type III polyketide synthase hybrid biosynthetic pathway for the structurally unique ansa compound kendomycin

Wenzel S, Bode H, Kochems I, Müller R (2008)

ChemBioChem, 9 (16): 2711-2721DOI: 10.1002/cbic.200800456

Crystal structure of a molecular assembly line

Weissman K, Müller R (2008)

Angew. Chem. Int. Ed. Engl., 47 (44): 8344-8346DOI: 10.1002/anie.200803293

Stereochemical determination and complex biosynthetic assembly of etnangien, a highly potent RNA polymerase inhibitor from the myxobacterium Sorangium cellulosum

Menche D, Arikan F, Perlova O, Horstmann N, Ahlbrecht W, Wenzel S, Jansen R, Irschik H, Müller R (2008)

J. Am. Chem. Soc., 130 (43): 14234-14243DOI: 10.1021/ja804194c

DKxanthene biosynthesis - understanding the basis for diversity-oriented synthesis in myxobacterial secondary metabolism

Meiser P, Weissman K, Bode H, Krug D, Dickschat J, Sandmann A, Müller R (2008)

Chem. Biol., 15 (8): 771-781DOI: 10.1016/j.chembiol.2008.06.005

Two functionally redundant Sfp-type 4'-phosphopantetheinyl transferases differentially activate biosynthetic pathways in Myxococcus xanthus

Meiser P, Müller R (2008)

ChemBioChem, 9 (10): 1549-1553DOI: 10.1002/cbic.200800077

Stereochemical determination of thuggacins A-C, highly active antibiotics from the myxobacterium Sorangium cellulosum

Bock M, Buntin K, Müller R, Kirschning A (2008)

Angew. Chem. Int. Ed. Engl., 47 (12): 2308-2311DOI: 10.1002/anie.200704897

Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition

Fu J, Wenzel S, Perlova O, Wang J, Gross F, Tang Z, Yin Y, Stewart A, Müller R, Zhang Y (2008)

Nucleic Acids Res., 36 (17)DOI: 10.1093/nar/gkn499

A transposon-based strategy to scale up myxothiazol production in myxobacterial cell factories

Sandmann A, Frank B, Müller R (2008)

J. Biotechnol., 135 (3): 255-261DOI: 10.1016/j.jbiotec.2008.05.001

Efficient mining of myxobacterial metabolite profiles enabled by liquid chromatography-electrospray ionization-time-of-flight mass spectrometry and compound-based principal component analysis

Krug D, Zurek G, Schneider B, Garcia R, Müller R (2008)

Anal. Chim. Acta, 624 (1): 97-106DOI: 10.1016/j.aca.2008.06.036

Myxochelin biosynthesis: direct evidence for two- and four-electron reduction of a carrier protein-bound thioester

Li Y, Weissman K, Müller R (2008)

J. Am. Chem. Soc., 130 (24): 7554-7555DOI: 10.1021/ja8025278

Heterologous expression and genetic engineering of the phenalinolactone biosynthetic gene cluster by using Red/ET recombineering

Binz T, Wenzel S, Schnell H, Bechthold A, Müller R (2008)

ChemBioChem, 9 (3): 447-454DOI: 10.1002/cbic.200700549

Protein-protein interactions in multienzyme megasynthetases

Weissman K, Müller R (2008)

ChemBioChem, 9 (6): 826-848DOI: 10.1002/cbic.200700751

Substrate specificity of the acyl transferase domains of EpoC from the epothilone polyketide synthase

Petkovic H, Sandmann A, Challis I, Hecht H, Silakowski B, Low L, Beeston N, Kuscer E, Garcia-Bernardo J, Leadlay P, …, Wilkinson B, Müller R (2008)

Org. Biomol. Chem., 6 (3): 500-506DOI: 10.1039/b714804f

Production of the antifungal isochromanone ajudazols A and B in Chondromyces crocatus Cm c5: biosynthetic machinery and cytochrome P450 modifications

Buntin K, Rachid S, Scharfe M, Blöcker H, Weissman K, Müller R (2008)

Angew. Chem. Int. Ed. Engl., 47 (24): 4595-4599DOI: 10.1002/anie.200705569

Discovering the Hidden Secondary Metabolome of Myxococcus xanthus: a Study of Intraspecific Diversity

Krug D, Zurek G, Revermann O, Vos M, Velicer G, Müller R (2008)

Appl. Environ. Microbiol., 74 (10): 3058-3068DOI: 10.1128/AEM.02863-07

2007

Complete genome sequence of the myxobacterium Sorangium cellulosum

Schneiker S, Perlova O, Kaiser O, Gerth K, Alici A, Altmeyer M, Bartels D, Bekel T, Beyer S, Bode E, …, Pühler A, Müller R (2007)

Nat. Biotechnol., 25 (11): 1281-1289DOI: 10.1038/nbt1354

From genetic diversity to metabolic unity: studies on the biosynthesis of aurafurones and aurafuron-like structures in myxobacteria and streptomycetes

Frank B, Wenzel S, Bode H, Scharfe M, Blöcker H, Müller R (2007)

J. Mol. Biol., 374 (1): 24-38DOI: 10.1016/j.jmb.2007.09.015

Mutasynthesis-derived myxalamids and origin of the isobutyryl-CoA starter unit of myxalamid B

Bode H, Meiser P, Klefisch T, Cortina N, Krug D, Göhring A, Schwär G, Mahmud T, Elnakady Y, Müller R (2007)

ChemBioChem, 8 (17): 2139-2144DOI: 10.1002/cbic.200700401

Mutational analysis of the myxovirescin biosynthetic gene cluster reveals novel insights into the functional elaboration of polyketide backbones

Simunovic V, Müller R (2007)

ChemBioChem, 8 (11): 1273-1280DOI: 10.1002/cbic.200700153

Biosynthesis of (R)-beta-tyrosine and its incorporation into the highly cytotoxic chondramides produced by Chondromyces crocatus

Rachid S, Krug D, Weissman K, Müller R (2007)

J. Biol. Chem., 282 (30): 21810-21817DOI: 10.1074/jbc.M703439200

Myxobacterial natural product assembly lines: fascinating examples of curious biochemistry

Wenzel S, Müller R (2007)

Nat. Prod. Rep., 24 (6): 1211-1224DOI: 10.1039/b706416k

Evidence for the mode of action of the highly cytotoxic Streptomyces polyketide kendomycin

Elnakady Y, Rohde M, Sasse F, Backes C, Keller A, Lenhof H, Weissman K, Müller R (2007)

ChemBioChem, 8 (11): 1261-1272DOI: 10.1002/cbic.200700050

SorF, a glycosyltransferase with promiscuous donor substrate specificity in vitro

Kopp M, Rupprath C, Irschik H, Bechthold A, Elling L, Müller R (2007)

ChemBioChem, 8 (7): 813-819DOI: 10.1002/cbic.200700024

Spiroketal polyketide formation in Sorangium: Identification and analysis of the biosynthetic gene cluster for the highly cytotoxic spirangienes

Frank B, Knauber J, Steinmetz H, Scharfe M, Blöcker H, Beyer S, Müller R (2007)

Chem. Biol., 14 (2): 221-233DOI: 10.1016/j.chembiol.2006.11.013

3-Hydroxy-3-methylglutaryl-CoA-like synthases direct the formation of methyl and ethyl side groups in the biosynthesis of the antibiotic myxovirescin A

Simunovic V, Müller R (2007)

ChemBioChem, 8 (5): 497-500DOI: 10.1002/cbic.200700017

Deciphering regulatory mechanisms for secondary metabolite production in the myxobacterium Sorangium cellulosum So ce56

Rachid S, Gerth K, Kochems I, Müller R (2007)

Mol. Microbiol., 63 (6): 1783-1796DOI: 10.1111/j.1365-2958.2007.05627.x

Reversible sugar transfer by glycosyltransferases as atool for natural product (bio)synthesis

Bode H, Müller R (2007)

Angew. Chem. Int. Ed. Engl., 46 (13): 2147-2150DOI: 10.1002/anie.200604671

A type II polyketide synthase from the gram-negative bacterium Stigmatella aurantiaca is involved in aurachin alkaloid biosynthesis

Sandmann A, Dickschat J, Jenke-Kodama H, Kunze B, Dittmann E, Müller R (2007)

Angew. Chem. Int. Ed. Engl., 46 (15): 2712-2716DOI: 10.1002/anie.200603513

2006

Analysis of myxobacterial secondary metabolism goes molecular

Bode H, Müller R (2006)

J. Ind. Microbiol. Biotechnol., 33 (7): 577-588DOI: 10.1007/s10295-006-0082-7

The unique DKxanthene secondary metabolite family from the myxobacterium Myxococcus xanthus is required for developmental sporulation

Meiser P, Bode H, Müller R (2006)

Proc. Natl. Acad. Sci. U.S.A., 103 (50): 19128-19133DOI: 10.1073/pnas.0606039103

Exploiting microbial diversity and bacterial genomics to find novel antibiotics

Müller R (2006)

Int. J. Med. Microbiol., 296S3 (42): 138-139

Metabolic engineering of Pseudomonas putida for methylmalonyl-CoA biosynthesis to enable complex heterologous secondary metabolite formation

Gross F, Ring M, Perlova O, Fu J, Schneider S, Gerth K, Kuhlmann S, Stewart A, Zhang Y, Müller R (2006)

Chem. Biol., 13 (12): 1253-1264DOI: 10.1016/j.chembiol.2006.09.014

Metabolic physiology of Pseudomonas putida for heterologous production of myxochromide

Stephan S, Heinzle E, Wenzel S, Krug D, Müller R, Wittmann C (2006)

Process Biochem., 41 (2006): 2146-2152DOI: 10.1016/j.procbio.2006.06.022

On the biosynthetic origin of methoxymalonyl-acyl carrier protein, the substrate for incorporation of 'glycolate' units into ansamitocin and soraphen A

Wenzel S, Williamson R, Grünanger C, Xu J, Gerth K, Martinez R, Moss S, Carroll B, Grond S, Unkefer C, Müller R, Floss H (2006)

J. Am. Chem. Soc., 128 (44): 14325-14336DOI: 10.1021/ja064408t

Proteome analysis of Myxococcus xanthus by off-line two-dimensional chromatographic separation using monolithic poly-(styrene-divinylbenzene) columns combined with ion-trap tandem mass spectrometry

Schley C, Altmeyer M, Swart R, Müller R, Huber C (2006)

J. Proteome Res., 5 (10): 2760-2768DOI: 10.1021/pr0602489

Biochemical characterization of MelJ and MelK - myxobacterial enzymes that transform an amide into a methyl ester

Müller I, Müller R (2006)

FEBS J., 273 (16): 3768-3778DOI: 10.1111/j.1742-4658.2006.05385.x

3-Hydroxy-3-methylglutaryl-coenzyme A (CoA) synthase is involved in the biosynthesis of isovaleryl-CoA in the myxobacterium Myxococcus xanthus during fruiting body formation

Bode H, Ring M, Schwär G, Kroppenstedt R, Kaiser D, Müller R (2006)

J. Bacteriol., 188 (18): 6524-6528DOI: 10.1128/JB.00825-06

A unique mechanism for methyl ester formation via an amide intermediate found in myxobacteria

Müller I, Weinig S, Steinmetz H, Kunze B, Veluthoor S, Mahmud T, Müller R (2006)

ChemBioChem, 7 (8): 1197-1205DOI: 10.1002/cbic.200600057

Reconstitution of myxothiazol biosynthetic gene cluster by Red/ET recombination and heterologous expression in Myxococcus xanthus

Perlova O, Fu J, Kuhlmann S, Krug D, Stewart F, Zhang Y, Müller R (2006)

Appl. Environ. Microbiol., 72 (12): 7485-7494DOI: 10.1128/AEM.01503-06

Genes and enzymes involved in caffeic acid biosynthesis in the actinomycete Saccharothrix espanaensis

Berner M, Krug D, Bihlmaier C, Vente A, Müller R, Bechthold A (2006)

J. Bacteriol., 188 (7): 2666-2673DOI: 10.1128/JB.188.7.2666–2673.2006

Myxovirescin biosynthesis is directed by hybrid polyketide synthases/ nonribosomal peptide synthetase, 3-hydroxy-3-methylglutaryl CoA synthases and trans-acting acyltransferases

Simunovic V, Zapp J, Rachid S, Krug D, Meiser P, Müller R (2006)

ChemBioChem, 7 (8): 1206-1220DOI: 10.1002/cbic.200600075

Development of simple media which allow investigations into the global regulation of chivosazol biosynthesis with Sorangium cellulosum So ce56

Müller R, Gerth K (2006)

J. Biotechnol., 121 (2): 192-200DOI: 10.1016/j.jbiotec.2005.10.012

Molecular and biochemical studies of chondramide formation - highly cytotoxic natural products from Chondromyces crocatus Cm c5

Rachid S, Krug D, Kochems I, Kunze B, Scharfe M, Blöcker H, Zabriski M, Müller R (2006)

Chem. Biol., 14: 667-681DOI: 10.1016/j.chembiol.2006.06.002

Establishment of a real-time PCR protocol for expression studies of secondary metabolite biosynthetic gene clusters in the G/C-rich myxobacterium Sorangium cellulosum So ce56

Kegler C, Gerth K, Müller R (2006)

J. Biotechnol., 121 (2): 201-212DOI: 10.1016/j.jbiotec.2005.10.007

Nonribosomal Peptide Biosynthesis: Point Mutations and Module Skipping Lead to Chemical Diversity

Wenzel S, Meiser P, Binz T, Mahmud T, Müller R (2006)

Angew. Chem. Int. Ed. Engl., 45 (14): 2296-2301DOI: 10.1002/anie.200503737

Identification of StiR, the first regulator of secondary metabolite formation in the myxobacterium Cystobacter fuscus Cb f17.1

Rachid S, Sasse F, Beyer S, Müller R (2006)

J. Biotechnol., 121 (4): 429-441DOI: 10.1016/j.jbiotec.2005.08.014

Bacterial type III polyketide synthases: Phylogenetic analysis and potential for the production of novel secondary metabolites by heterologous expression in pseudomonads

Gross F, Luniak N, Perlova O, Gaitatzis N, Jenke-Kodama H, Gerth K, Gottschalk D, Dittmann E, Müller R (2006)

Arch. Microbiol., 185 (1): 28-38DOI: 10.1007/s00203-005-0059-3

Identification and analysis of the chivosazol biosynthetic gene cluster from the myxobacterial model strain Sorangium cellulosum So ce56

Perlova O, Gerth K, Hans A, Kaiser O, Müller R (2006)

J. Biotechnol., 121 (2): 174-191DOI: 10.1016/j.jbiotec.2005.10.011

2005

Moderately thermophilic myxobacteria: novel potential for the production of natural products isolation and characterization

Gerth K, Müller R (2005)

Environ. Microbiol., 7 (6): 874-880DOI: 10.1111/j.1462-2920.2005.00761.x

Heterologe Expression von komplexen myxobakteriellen Naturstoff-Biosynthesewegen in Pseudomonaden

Wenzel S, Müller R (2005)

Biospektrum, 11 (5): 628-631

Recent developments towards the heterologous expression of complex bacterial natural product biosynthetic pathways

Wenzel S, Müller R (2005)

Curr. Opin. Biotechnol., 16 (6): 594-606DOI: 10.1016/j.copbio.2005.10.001

Evolutionary implications of bacterial polyketide synthases

Jenke-Kodama H, Sandmann A, Müller R, Dittmann E (2005)

Mol. Biol. Evol., 22 (10): 2027-2039DOI: 10.1093/molbev/msi193

The impact of bacterial genomics on natural product research

Bode H, Müller R (2005)

Angew. Chem. Int. Ed. Engl., 44 (42): 6828-6846DOI: 10.1002/anie.200501080

Production of the tubulin destabilizer disorazol in Sorangium cellulosum: biosynthetic machinery and regulatory genes

Kopp M, Irschik H, Pradella S, Müller R (2005)

ChemBioChem, 6 (7): 1277-1286DOI: 10.1002/cbic.200400459

Formation of novel secondary metabolites by bacterial multimodular assembly lines: deviations from text book biosynthetic logic

Wenzel S, Müller R (2005)

Curr. Opin. Chem. Biol., 9: 447-458DOI: 10.1016/j.cbpa.2005.08.001

Aurafuron A and B, new bioactive polyketides from Stigmatella aurantiaca and Archangium gephyra (myxobacteria)

Kunze B, Reichenbach H, Müller R, Höfle G (2005)

J. Antibiot., 58 (4): 244-251

A novel type of geosmin biosynthesis in myxobacteria

Dickschat J, Bode H, Mahmud T, Müller R, Schulz S (2005)

J. Org. Chem., 70 (13): 5174-5182DOI: 10.1021/jo050449g

Novel insights into siderophore formation in myxobacteria

Gaitatzis N, Kunze B, Müller R (2005)

ChemBioChem, 6 (2): 365-374DOI: 10.1002/cbic.200400206

Heterologous expression of a myxobacterial natural products assembly line in pseudomonads via red/ET recombineering

Wenzel S, Gross F, Zhang Y, Fu J, Stewart F, Müller R (2005)

Chem. Biol., 12 (3): 349-356DOI: 10.1016/j.chembiol.2004.12.012

Structure and Biosynthesis of Myxochromides S1-3 in Stigmatella aurantiaca: Evidence for An Iterative Bacterial Type I Polyketide Synthase and for Module Skipping in Nonribosomal Peptide Biosynthesis

Wenzel S, Kunze B, Höfle G, Silakowski B, Scharfe M, Blöcker H, Müller R (2005)

ChemBioChem, 6 (2): 375-385DOI: 10.1002/cbic.200400282

Posttranslational modification of myxobacterial carrier protein domains in Pseudomonas sp. by an intrinsic phosphopantetheinyl transferase

Gross F, Gottschalk D, Müller R (2005)

Appl. Microbiol. Biotechnol., 68 (1): 66-74DOI: 10.1007/s00253-004-1836-7

Biosynthesis of iso-fatty acids in myxobacteria: iso-even fatty acids are derived by alpha-oxidation of iso-odd fatty acids

Bode H, Dickschat J, Kroppenstedt R, Schulz S, Müller R (2005)

J. Am. Chem. Soc., 127 (2): 532-533DOI: 10.1021/ja043570y

A novel biosynthetic pathway to isovaleryl-CoA in myxobacteria: The involvement of the mevalonate pathway

Mahmud T, Wenzel S, Wan E, Wen K, Bode H, Gaitatzis N, Müller R (2005)

ChemBioChem, 6 (2): 322-330DOI: 10.1002/cbic.200400261

2004

Methods for heterologous expression of secondary metabolites

Wenzel S, Gross F, Zhang Y, Stewart F, Müller R (2004)

Patent

Identification and analysis of the core biosynthetic machinery of tubulysin, a potent cytotoxin with potential anticancer activity

Sandmann A, Sasse F, Müller R (2004)

Chem. Biol., 11 (8): 1071-1079DOI: 10.1016/j.chembiol.2004.05.014

Unusual biosynthesis of leupyrrins in the myxobacterium Sorangium cellulosum

Bode H, Wenzel S, Irschik H, Höfle G, Müller R (2004)

Angew. Chem. Int. Ed. Engl., 43 (32): 4163-4167DOI: 10.1002/anie.200454240

Biosynthesis of volatiles by the myxobacterium Myxococcus xanthus

Dickschat J, Wenzel S, Bode H, Müller R, Schulz S (2004)

ChemBioChem, 5 (6): 778-787DOI: 10.1002/cbic.200300813

Don't classify polyketide synthases

Müller R (2004)

Chem. Biol., 11 (1): 4-6DOI: 10.1016/j.chembiol.2004.01.005

Critical variations of conjugational DNA transfer into secondary metabolite multiproducing Sorangium cellulosum strains So ce12 and So ce56: development of a mariner-based transposon mutagenesis system

Kopp M, Irschik H, Gross F, Perlova O, Sandmann A, Gerth K, Müller R (2004)

J. Biotechnol., 107 (1): 29-40DOI: 10.1016/j.jbiotec.2003.09.013

2003

Melithiazol biosynthesis: further insights into myxobacterial PKS/NRPS systems and evidence for a new subclass of methyl transferases

Weinig S, Hecht H, Mahmud T, Müller R (2003)

Chem. Biol., 10 (10): 939-952DOI: 10.1016/j.chembiol.2003.09.012

Markerless mutations in the myxothiazol biosynthetic gene cluster: A delicate megasynthetase with a superfluous nonribosomal peptide synthetase domain

Weinig S, Mahmud T, Müller R (2003)

Chem. Biol., 10 (10): 953-960DOI: 10.1016/j.chembiol.2003.09.013

Myxobacteria: proficient producers of novel natural products with various biological activities - past and future biotechnological aspects with the focus on the genus Sorangium

Gerth K, Pradella S, Perlova O, Beyer S, Müller R (2003)

J. Biotechnol., 106: 233-253DOI: 10.1016/j.jbiotec.2003.07.015

The leupyrrins: A structurally unique family of secondary metabolites from the myxobacterium Sorangium cellulosum

Bode H, Irschik H, Wenzel S, Reichenbach H, Müller R, Höfle G (2003)

J. Nat. Prod., 66 (9): 1203-1206DOI: 10.1021/np030109v

Possibility of bacterial recruitment of plant genes associated with the biosynthesis of secondary metabolites

Bode H, Müller R (2003)

Plant Physiol., 132 (3): 1153-1161DOI: 10.1104/pp.102.019760.

Steroid biosynthesis in prokaryotes: identification of myxobacterial steroids and cloning of the first bacterial 2,3(S)-oxidosqualene cyclase from the myxobacterium Stigmatella aurantiaca

Bode H, Zeggel B, Silakowski B, Wenzel S, Reichenbach H, Müller R (2003)

Mol. Microbiol., 47 (2): 471-481DOI: 10.1046/j.1365-2958.2003.03309.x

2002

A novel biosynthetic pathway providing precursors for fatty acid biosynthesis and secondary metabolite formation in myxobacteria

Mahmud T, Bode H, Silakowski B, Kroppenstedt R, Xu M, Nordhoff S, Höfle G, Müller R (2002)

J. Biol. Chem., 277 (36): 32768-74DOI: 10.1074/jbc.M205222200

The biosynthesis of the aromatic myxobacterial electron transport inhibitor stigmatellin is directed by a novel type of modular polyketide synthase

Gaitatzis N, Silakowski B, Kunze B, Nordsiek G, Blöcker H, Höfle G, Müller R (2002)

J. Biol. Chem., 277 (15): 13082-13090DOI: 10.1074/jbc.M111738200

2001

In vitro reconstitution of the myxochelin biosynthetic machinery of Stigmatella aurantiaca Sg a15: Biochemical characterization of a reductive release mechanism from nonribosomal peptide synthetases

Gaitatzis N, Kunze B, Müller R (2001)

Proc. Natl. Acad. Sci. USA, 98 (20): 11136-11141DOI: 10.1073/pnas.201167098

The mtaA gene of the myxothiazol biosynthetic gene cluster from Stigmatella aurantiaca DW4/3-1 encodes a phosphopantetheinyl transferase that activates polyketide synthases and polypeptide synthetases

Gaitatzis N, Hans A, Müller R, Beyer S (2001)

J. Biochem. (Tokyo), 129 (1): 119-124

Novel features in a combined polyketide synthase/non-ribosomal peptide synthetase: the myxalamid biosynthetic gene cluster of the myxobacterium Stigmatella aurantiaca Sga15

Silakowski B, Nordsiek G, Kunze B, Blöcker H, Müller R (2001)

Chem. Biol., 8 (1): 59-69DOI: 10.1016/S1074-5521(00)00056-9

Multiple hybrid polyketide synthase/non-ribosomal peptide synthetase gene clusters in the myxobacterium Stigmatella aurantiaca

Silakowski B, Kunze B, Müller R (2001)

Gene, 275 (2): 233-240

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