Drug Delivery Across Biological Barriers
Prof Dr Claus-Michael Lehr
The research focus of Prof. Lehr’s group is on new technologies for the safe and efficient delivery of anti-infectives across biological barriers, such as the lungs, the intestinal mucosa and the skin, but also mucus, biofilms and the bacterial cell envelope.
Our research and approach
Advances in molecular biotechnology and medicinal chemistry are continuously generating new drug candidates. However, developing these molecules into successful therapeutics also requires optimizing their biopharmaceutical properties, in particular the ability to cross biological barriers, as represented by tissues, cells and cellular membranes. As further optimizing the active compounds is often not possible, new technologies are needed to ensure safe and effective delivery to the site of action, ideally by non-invasive (“needle-free”) administration. In this context we are working both on innovative drug carriers as well as on predictive in vitro models with the aim to accelerate preclinical development and to facilitate clinical translation.
Team members

Prof Dr Claus-Michael Lehr
Group Leader

Annette Herkströter
Secretary

Dr Brigitta Loretz
Scientist

Cristiane de Souza Carvalho Wodarz
Scientist

Dr Alberto Hidalgo
Postdoc

Dr Sangeun Lee
Postdoc

Thiyagarajan Durairaj
Postdoc

Benedikt Constantin Huck
PhD Student

Clémentine Richter
PhD Student

Lena Kliesch
PhD Student

Patrick Carius
PhD Student

Rebekka Christmann
PhD Student

Robert Richter
PhD Student

Sara Nasr
PhD Student
Research projects
LUNG-ON-A-CHIP: Bioengineered microfluidic in vitro test system to study alveolar transport and clearance
The aim of the project focuses on the design and integration of an innovative bioengineered microfluidic system that mimics the characteristic physiological microenvironment as well as the unique cellular composition of the lung. By reconstituting the human alveolar epithelial barrier in vitro, our organ-on-chip systems serve as an integrative platform for in vitro analysis of both, transport across the air-blood-barrier into the blood stream as well as uptake and subsequent clearance by alveolar macrophages. Such model is expected to have significant impact for predictive preclinical characterisation of advanced aerosol medicines, in healthy as well as in diseased state.
ANTI-TB: Antibiotika-beladene Nanocarrier zur Therapeutischen Inhalation gegen Tuberkulose
The ANTI-TB project aims to develop a novel therapy for tuberculosis. Tuberculosis is the world's most serious bacterial infection in humans. Their control is hampered by the rising occurrence of therapy-resistant M tuberculosis strains. The project uses a nanomedical approach to improve the unsatisfying therapy situation, in particular for infections with therapy-resistant pathogens, and to reduce further resistance developments. Nanocarriers will be combined with new anti-tuberculosis drugs and optimized with specific surface modifications that direct them to infection sites, infected cells, and pathogens. For ease of use, the most effective antibiotic nanocarriers will ultimately be converted into an aerosol or powder inhalation therapy. The strategy aims to increase the local drug concentrations in the lung and shorten the therapy duration. Such an approach should minimize the onset of drug-resistant pathogens, reduce systemic side effects, and improve patients' preparedness for therapy. The HIPS investigates the effects and interactions of the nanoparticles with the lungs' physiological fluids, mucus and lung surfactant, as well as macrophages and epithelial cells. The aerosol powder generation is also our task.
Project website: http://antitb.fz-borstel.de/index.php/en/

NEW DEAL: New nanotherapy research for inflammatory bowel diseases
The NEW DEAL project aims to develop a highly selective and local therapy with an improved efficacy and safety profile for IBD patients. Specific siRNAs targeting Jak-1 or Jak-3, nanoparticle carriers, and formulations for oral application are developed. This treatment might be an alternative to the existing therapies. In this project, the HIPS is contributing with its expertise on advanced coculture models of the inflamed intestinal barrier and the mucus. We investigate the intestinal barrier crossing of the nanocarriers.
Project website: https://newdeal-project.eu/

Publications
2020
PLGA nanocapsules improve the delivery of clarithromycin to kill intracellular Staphylococcus aureus and Mycobacterium abscessus
Anversa Dimer F, Souza Carvalho-Wodarz C, Goes A, Cirnski K, Herrmann J, Schmitt V, Pätzold L, Abed N, Rossi C, Bischoff M, …, Müller R, Lehr C (2020)
Nanomedicine : nanotechnology, biology, and medicine, 24DOI: 10.1016/j.nano.2019.102125
Advancing human in vitro pulmonary disease models in preclinical research: opportunities for lung-on-chips
Artzy-Schnirman A, Lehr C, Sznitman J (2020)
Expert Opinion on Drug Delivery, 17 (5): 621-625DOI: 10.1080/17425247.2020.1738380
P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
Montefusco-Pereira C, Horstmann J, Ebensen T, Beisswenger C, Bals R, Guzmán C, Schneider-Daum N, Carvalho-Wodarz C, Lehr C (2020)
Journal of visualized experiments : JoVE (160)DOI: 10.3791/61069
Safety assessment of excipients (SAFE) for orally inhaled drug products
Metz J, Scharnowske L, Hans F, Schnur S, Knoth K, Zimmer H, Limberger M, Groß H, Lehr C, Hittinger M (2020)
ALTEX, 37 (2): 275-286DOI: 10.14573/altex.1910231
Nanoparticle Targeting to Scalp Hair Follicles: New Perspectives for a Topical Therapy for Alopecia Areata
Christmann R, Thomas C, Jager N, Raber A, Loretz B, Schaefer U, Tschernig T, Vogt T, Lehr C (2020)
The Journal of investigative dermatology, 140 (1): 243-246DOI: 10.1016/j.jid.2019.05.028
pH-Dependent morphology and optical properties of lysine-derived molecular biodynamers
Lee S, Kaya C, Jang H, Koch M, Loretz B, Buhler E, Lehr C, Hirsch A (2020)
Mater. Chem. Front., 4 (3): 905-909DOI: 10.1039/C9QM00651F
Itaconic Acid Increases the Efficacy of Tobramycin against Pseudomonas aeruginosa Biofilms
Ho D, Rossi C, Loretz B, Murgia X, Lehr C (2020)
Pharmaceutics, 12 (8)DOI: 10.3390/pharmaceutics12080691
Squalenyl Hydrogen Sulfate Nanoparticles for Simultaneous Delivery of Tobramycin and an Alkylquinolone Quorum Sensing Inhibitor Enable the Eradication of P. aeruginosa Biofilm Infections
Ho D, Murgia X, Rossi C, Christmann R, Hüfner de Mello Martins A, Koch M, Andreas A, Herrmann J, Müller R, Empting M, …, Couvreur P, Lehr C (2020)
Angewandte Chemie (International ed. in English)DOI: 10.1002/anie.202001407
Design and in vitro characterization of multistage silicon-PLGA budesonide particles for inflammatory bowel disease
Leonard F, Srinivasan S, Liu X, Collnot E, Ferrari M, Lehr C, Godin B (2020)
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 151: 61-72DOI: 10.1016/j.ejpb.2020.03.020
3D air-liquid interface culture of Cystic Fibrosis bronchial epithelia, macrophages and P. aeruginosa to assess host-pathogen interaction and drug efficacy
Montefusco C, Montefusco-Pereira C (2020)
BookDOI: 10.22028/D291-31815
Inhalationstoxizität von Nanopartikeln // The role of alveolar macrophages in biokinetics and biological effects of inhaled nanoparticles
Koltermann-Jülly J (2020)
BookDOI: 10.22028/D291-31034
Coupling quaternary ammonium surfactants to the surface of liposomes improves both antibacterial efficacy and host cell biocompatibility
Montefusco-Pereira C, Formicola B, Goes A, Re F, Marrano C, Mantegazza F, Carvalho-Wodarz C, Fuhrmann G, Caneva E, Nicotra F, Lehr C, Russo L (2020)
European Journal of Pharmaceutics and Biopharmaceutics, 149: 12-20DOI: 10.1016/j.ejpb.2020.01.013
Streptococcal Extracellular Membrane Vesicles Are Rapidly Internalized by Immune Cells and Alter Their Cytokine Release
Mehanny M, Koch M, Lehr C, Fuhrmann G (2020)
Frontiers in immunology, 11DOI: 10.3389/fimmu.2020.00080
2019
Triple co-culture of human alveolar epithelium, endothelium and macrophages for studying the interaction of nanocarriers with the air-blood barrier
Costa A, Souza Carvalho-Wodarz C, Seabra V, Sarmento B, Lehr C (2019)
Acta BiomaterialiaDOI: 10.1016/j.actbio.2019.04.037
Challenges and Strategies in Drug Delivery Systems for Treatment of Pulmonary Infections
Ho D, Nichols B, Edgar K, Murgia X, Loretz B, Lehr C (2019)
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.VDOI: 10.1016/j.ejpb.2019.09.002
Advanced in vitro lung-on-chip platforms for inhalation assays: From prospect to pipeline
Artzy-Schnirman A, Hobi N, Schneider-Daum N, Guenat O, Lehr C, Sznitman J (2019)
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 144: 11-17DOI: 10.1016/j.ejpb.2019.09.006
Preferential uptake of chitosan-coated PLGA nanoparticles by primary human antigen presenting cells
Durán V, Yasar H, Becker J, Thiyagarajan D, Loretz B, Kalinke U, Lehr C (2019)
Nanomedicine : nanotechnology, biology, and medicine, 21DOI: 10.1016/j.nano.2019.102073
Mechanistic profiling of the release kinetics of siRNA from lipidoid-polymer hybrid nanoparticles in vitro and in vivo after pulmonary administration
Thanki K, van Eetvelde D, Geyer A, Fraire J, Hendrix R, van Eygen H, Putteman E, Sami H, Souza Carvalho-Wodarz C, Franzyk H, …, Ogris M, Foged C (2019)
Journal of controlled release : official journal of the Controlled Release Society, 310: 82-93DOI: 10.1016/j.jconrel.2019.08.004
OCTN2-Mediated Acetyl-l-Carnitine Transport in Human Pulmonary Epithelial Cells In Vitro
Salomon J, Gausterer J, Selo M, Hosoya K, Huwer H, Schneider-Daum N, Lehr C, Ehrhardt C (2019)
Pharmaceutics, 11 (8)DOI: 10.3390/pharmaceutics11080396
Preparation, characterization and in vitro antibacterial property of Ciprofloxacin-loaded nanostructured lipid carrier for treatment of Bacillus subtilis infection
Nnamani P, Ugwu A, Ibezim E, Onoja S, Odo A, Windbergs M, Rossi C, Lehr C, Attama A (2019)
Journal of Microencapsulation: 1-30DOI: 10.1080/02652048.2019.1582724
Capturing the Onset of Bacterial Pulmonary Infection in Acini‐On‐Chips
Artzy-Schnirman A, Zidan H, Elias-Kirma S, Ben-Porat L, Tenenbaum-Katan J, Carius P, Fishler R, Schneider-Daum N, Lehr C, Sznitman J (2019)
Adv. Biosys., 3 (9)DOI: 10.1002/adbi.201900026
The synergistic effect of chlorotoxin-mApoE in boosting drug-loaded liposomes across the BBB
Formicola B, Dal Magro R, Montefusco-Pereira C, Lehr C, Koch M, Russo L, Grasso G, Deriu M, Danani A, Bourdoulous S, Re F (2019)
Journal of nanobiotechnology, 17 (1)DOI: 10.1186/s12951-019-0546-3
Macro- and Microrheological Properties of Mucus Surrogates in Comparison to Native Intestinal and Pulmonary Mucus
Huck B, Hartwig O, Biehl A, Schwarzkopf K, Wagner C, Loretz B, Murgia X, Lehr C (2019)
Biomacromolecules, 20 (9): 3504-3512DOI: 10.1021/acs.biomac.9b00780
Bioinspired Liposomes for Oral Delivery of Colistin to Combat Intracellular Infections by Salmonella enterica
Menina S, Eisenbeis J, Kamal M, Koch M, Bischoff M, Gordon S, Loretz B, Lehr C (2019)
Advanced healthcare materials, 8 (17)DOI: 10.1002/adhm.201900564
Pseudobacterial nanocarriers for intracellular delivery of anti-infectives
Castoldi A (2019)
UnpublishedWorkDOI: 10.22028/D291-27989
Non-invasive vaccination by nanoparticle-based messenger RNA (mRNA) delivery via the transfollicular route
Yasar H (2019)
UnpublishedWorkDOI: 10.22028/D291-27933
Interaction of nanoparticles with the pulmonary barrier; The role of mucus in health and disease // The Role of Airway Mucus as a Barrier to Nanoparticles: Implications for Pulmonary Drug Delivery
Murgia X, Murgia Esteve X (2019)
BookDOI: 10.22028/D291-31240
2018
Kinetics of mRNA delivery and protein translation in dendritic cells using lipid-coated PLGA nanoparticles
Yasar H, Biehl A, Rossi C, Koch M, Murgia X, Loretz B, Lehr C (2018)
Journal of nanobiotechnology, 16 (1)DOI: 10.1186/s12951-018-0401-y
Oral delivery of macromolecular drugs
Savla R, Hartwig O, Lim Chin W, Loretz B, Lehr C (2018)
Pharm. Technol. Eur., 30 (7): 22-26
The role of mucus on drug transport and its potential to affect therapeutic outcomes
Murgia X, Loretz B, Hartwig O, Hittinger M, Lehr C (2018)
Advanced Drug Delivery Reviews, 124: 82-97DOI: 10.1016/j.addr.2017.10.009
Can lifecycle management safeguard innovation in the pharmaceutical industry?
Hering S, Loretz B, Friedli T, Lehr C, Stieneker F (2018)
Drug Discovery TodayDOI: 10.1016/j.drudis.2018.10.008
Farnesylated Glycol Chitosan as a Platform for Drug Delivery
Ho D, Frisch S, Biehl A, Terriac E, Rossi C, Schwarzkopf K, Lautenschläger F, Loretz B, Murgia X, Lehr C (2018)
Biomacromolecules, 19 (8): 3489-3501DOI: 10.1021/acs.biomac.8b00795
Chemically modified h CFTR mRNAs recuperate lung function in a mouse model of cystic fibrosis
Haque, A. K. M. Ashiqul, Dewerth A, Antony J, Riethmüller J, Schweizer G, Weinmann P, Latifi N, Yasar H, Pedemonte N, …, Handgretinger R, Kormann M (2018)
Scientific Reports, 8 (1)DOI: 10.1038/s41598-018-34960-0
Postmenopausaler Lichen planopilaris alias fibrosierende frontotemporale Alopezie Kossard
Vogt T, Thomas C, Reichrath J, Schilling L, Mawlood D, Christmann R, Loretz B, Schäfer U, Lehr C, Müller C (2018)
Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete, 69 (2): 134-142DOI: 10.1007/s00105-017-4118-x
Murine alveolar epithelial cells and their lentivirus-mediated immortalisation
Sapich S, Hittinger M, Hendrix-Jastrzebski R, Repnik U, Griffiths G, May T, Wirth D, Bals R, Schneider-Daum N, Lehr C (2018)
Alternatives to laboratory animals : ATLA, 46 (2): 73-89
Medium throughput breathing human primary cell alveolus-on-chip model
Stucki J, Hobi N, Galimov A, Stucki A, Schneider-Daum N, Lehr C, Huwer H, Frick M, Funke-Chambour M, Geiser T, Guenat O (2018)
Scientific Reports, 8 (1)DOI: 10.1038/s41598-018-32523-x
Towards Standardized Dissolution Techniques for In Vitro Performance Testing of Dry Powder Inhalers
Floroiu A, Klein M, Krämer J, Lehr C (2018)
Dissolution Technologies, 25 (3): 6-18DOI: 10.14227/DT250318P6
Starch-Chitosan Polyplexes
Yasar H, Ho D, Rossi C, Herrmann J, Gordon S, Loretz B, Lehr C (2018)
Polymers, 10 (3)DOI: 10.3390/polym10030252
Co-culture of human alveolar epithelial (hAELVi) and macrophage (THP-1) cell lines
Kletting S, Barthold S, Repnik U, Griffiths G, Loretz B, Schneider-Daum N, Souza Carvalho-Wodarz C, Lehr C (2018)
ALTEX, 35 (2): 211-222DOI: 10.14573/altex.1607191
A miRNA181a/NFAT5 axis links impaired T cell tolerance induction with autoimmune type 1 diabetes
Serr I, Scherm M, Zahm A, Schug J, Flynn V, Hippich M, Kälin S, Becker M, Achenbach P, Nikolaev A, …, Ziegler A, Daniel C (2018)
Science translational medicine, 10 (422)DOI: 10.1126/scitranslmed.aag1782
Human airway mucus alters susceptibility of Pseudomonas aeruginosa biofilms to tobramycin, but not colistin
Müller L, Murgia X, Siebenbürger L, Börger C, Schwarzkopf K, Sewald K, Häussler S, Braun A, Lehr C, Hittinger M, Wronski S (2018)
The Journal of antimicrobial chemotherapy, 73 (10): 2762-2769DOI: 10.1093/jac/dky241
Combining MucilAir™ and Vitrocell® Powder Chamber for the In Vitro Evaluation of Nasal Ointments in the Context of Aerosolized Pollen
Metz J, Knoth K, Groß H, Lehr C, Stäbler C, Bock U, Hittinger M (2018)
Pharmaceutics, 10 (2)DOI: 10.3390/pharmaceutics10020056
Polysaccharide Submicrocarrier for Improved Pulmonary Delivery of Poorly Soluble Anti-infective Ciprofloxacin
Ho D, Costa A, Rossi C, Souza Carvalho-Wodarz C, Loretz B, Lehr C (2018)
Molecular pharmaceutics, 15 (3): 1081-1096DOI: 10.1021/acs.molpharmaceut.7b00967
In Vitro Model of the Gram-Negative Bacterial Cell Envelope for Investigation of Anti-Infective Permeation Kinetics
Graef F, Richter R, Fetz V, Murgia X, Rossi C, Schneider-Daum N, Allegretta G, Elgaher W, Haupenthal J, Empting M, …, Gordon S, Lehr C (2018)
ACS infectious diseases, 4 (8): 1188-1196DOI: 10.1021/acsinfecdis.7b00165
Vibrational spectroscopic imaging and live cell video microscopy for studying differentiation of primary human alveolar epithelial cells
Vukosavljevic B, Hittinger M, Hachmeister H, Pilger C, Murgia X, Gepp M, Gentile L, Huwer H, Schneider-Daum N, Huser T, Lehr C, Windbergs M (2018)
Journal of biophotonicsDOI: 10.1002/jbio.201800052
A novel co-culture model of human bronchial epithelial cells and Pseudomonas aeruginosa biofilms to mimic chronic lung infections in cystic fibrosis
Juntke J (2018)
UnpublishedWorkDOI: 10.22028/D291-27897
Novel anti-infective delivery systems for the treatment of pulmonary bacterial infections
Ho D (2018)
UnpublishedWorkDOI: 10.22028/D291-27854
Aspherical and Spherical InvA497-Functionalized Nanocarriers for Intracellular Delivery of Anti-Infective Agents
Castoldi A, Empting M, Rossi C, Mayr K, Dersch P, Hartmann R, Müller R, Gordon S, Lehr C (2018)
Pharm Res, 36 (1)DOI: 10.1007/s11095-018-2521-3
Extracellular vesicles protect glucuronidase model enzymes during freeze-drying
Frank J, Richter M, Rossi C, Lehr C, Fuhrmann K, Fuhrmann G (2018)
Scientific Reports, 8 (1)DOI: 10.1038/s41598-018-30786-y
2017
Permanent gene correction by means of nucleotide-modified messenger RNA
Kormann M, Lehr C, Loretz B, Malaeksefat E (2017)
Patent (DE/08.10.13/ DEA102013111099)
Cell and tissue-based in vitro models for improving the development of oral inhalation drug products
Hittinger M, Schneider-Daum N, Lehr C (2017)
European Journal of Pharmaceutics and Biopharmaceutics, 118: 73-78DOI: 10.1016/j.ejpb.2017.02.019
Ciprofloxacin-loaded PLGA nanoparticles against cystic fibrosis P. aeruginosa lung infections
Günday Türeli N, Torge A, Juntke J, Schwarz B, Schneider-Daum N, Türeli A, Lehr C, Schneider M (2017)
European Journal of Pharmaceutics and Biopharmaceutics, 117: 363-371DOI: 10.1016/j.ejpb.2017.04.032
A foam model highlights the differences of the macro- and microrheology of respiratory horse mucus
Gross A, Torge A, Schaefer U, Schneider M, Lehr C, Wagner C (2017)
Journal of the Mechanical Behavior of Biomedical Materials, 71: 216-222DOI: 10.1016/j.jmbbm.2017.03.009
A Model for the Transient Subdiffusive Behavior of Particles in Mucus
Ernst M, John T, Guenther M, Wagner C, Schaefer U, Lehr C (2017)
Biophysical journal, 112 (1): 172-179DOI: 10.1016/j.bpj.2016.11.900
Calcifediol-loaded liposomes for local treatment of pulmonary bacterial infections
Castoldi A, Herr C, Niederstraßer J, Labouta H, Melero A, Gordon S, Schneider-Daum N, Bals R, Lehr C (2017)
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 118: 62-67DOI: 10.1016/j.ejpb.2016.11.026
Biodegradable starch derivatives with tunable charge density-synthesis, characterization, and transfection efficiency
Thiele C, Loretz B, Lehr C (2017)
Drug delivery and translational research, 7 (2): 252-258DOI: 10.1007/s13346-016-0333-8
Tracing molecular and structural changes upon mucolysis with N-acetyl cysteine in human airway mucus
Vukosavljevic B, Murgia X, Schwarzkopf K, Schaefer U, Lehr C, Windbergs M (2017)
International journal of pharmaceutics, 533 (2): 373-376DOI: 10.1016/j.ijpharm.2017.07.022
Expression and Activity of Breast Cancer Resistance Protein (BCRP/ABCG2) in Human Distal Lung Epithelial Cells In Vitro
Nickel S, Selo M, Fallack J, Clerkin C, Huwer H, Schneider-Daum N, Lehr C, Ehrhardt C (2017)
Pharmaceutical researchDOI: 10.1007/s11095-017-2172-9
Targeted delivery of Cyclosporine A by polymeric nanocarriers improves the therapy of inflammatory bowel disease in a relevant mouse model
Melero A, Draheim C, Hansen S, Giner E, Carreras J, Talens-Visconti R, Garrigues T, Peris J, Recio M, Giner R, Lehr C (2017)
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 119: 361-371DOI: 10.1016/j.ejpb.2017.07.004
Modelling the bronchial barrier in pulmonary drug delivery
Murgia X, Yasar H, Carvalho-Wodarz C, Loretz B, Gordon S, Schwarzkopf K, Schaefer U, Lehr C (2017)
European Journal of Pharmaceutics and Biopharmaceutics, 118: 79-88DOI: 10.1016/j.ejpb.2017.03.020
Nanoencapsulation of a glucocorticoid improves barrier function and anti-inflammatory effect on monolayers of pulmonary epithelial cell lines
Rigo L, Carvalho-Wodarz C, Pohlmann A, Guterres S, Schneider-Daum N, Lehr C, Beck R (2017)
European Journal of Pharmaceutics and BiopharmaceuticsDOI: 10.1016/j.ejpb.2017.05.006
Diverse Applications of Nanomedicine
Pelaz B, Alexiou C, Alvarez-Puebla R, Alves F, Andrews A, Ashraf S, Balogh L, Ballerini L, Bestetti A, Brendel C, …, Zhou X, Parak W (2017)
ACS Nano, 11 (3): 2313-2381DOI: 10.1021/acsnano.6b06040
Lung alveoli array-on-chip with a bioartificial membrane
Zamprogno P, Achenbach S, Stucki J, Hobi N, Schneider-Daum N, Lehr C, Huwer H, Schmid R, Guenat O (2017)
Toxicology letters, 280DOI: 10.1016/j.toxlet.2017.07.974
Modeling the Gram-negative bacterial cell envelope
Gräf F (2017)
Book
Sustained drug delivery for local treatment of joint diseases
Sterner B (2017)
Book
Autologous coculture of primary human alveolar epithelial cells and macrophages for evaluating the safety and efficacy of novel inhalation pharmaceuticals
Hittinger M (2017)
Book
Modeling and simulation of the interaction between particles and pulmonary mucus
Ernst M (2017)
Book
Interleukin-10-Loaded Nano- and Microparticles for the Local Treatment of the Intestinal Mucosa and the Deep Lung
Nico Alexander Mell (2017)
2016
Anti-infectives in Drug Delivery-Overcoming the Gram-Negative Bacterial Cell Envelope
Graef F, Gordon S, Lehr C (2016)
Current topics in microbiology and immunology, 398: 475-496DOI: 10.1007/82_2016_491
New Horizons in the Development of Novel Needle-Free Immunization Strategies to Increase Vaccination Efficacy
Schulze K, Ebensen T, Riese P, Prochnow B, Lehr C, Guzman C (2016)
Current topics in microbiology and immunologyDOI: 10.1007/82_2016_495
The bacterial cell envelope as delimiter of anti-infective bioavailability – An in vitro permeation model of the Gram-negative bacterial inner membrane
Graef F, Vukosavljevic B, Michel J, Wirth M, Ries O, De Rossi C, Windbergs M, Rosilio V, Ducho C, Gordon S, Lehr C (2016)
Journal of Controlled Release, 243: 214-224DOI: 10.1016/j.jconrel.2016.10.018
Calcium Phosphate System for Gene Delivery: Historical Background and Emerging Opportunities
Mostaghaci B, Loretz B, Lehr C (2016)
Current Pharmaceutical Design, 22 (11): 1529-33
In vitro models for evaluating safety and efficacy of novel technologies for skin drug delivery
Planz V, Lehr C, Windbergs M (2016)
Journal of Controlled Release, 242: 89-104DOI: 10.1016/j.jconrel.2016.09.002
miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity
Serr I, Furst R, Ott V, Scherm M, Nikolaev A, Gokmen F, Kalin S, Zillmer S, Bunk M, Weigmann B, …, Ziegler A, Daniel C (2016)
Proceedings of the National Academy of Sciences of the United States of America, 113 (43): 6659-DOI: 10.1073/pnas.1606646113
IL-17A-mediated expression of epithelial IL-17C promotes inflammation during acute Pseudomonas aeruginosa pneumonia
Wolf L, Sapich S, Honecker A, Jungnickel C, Seiler F, Bischoff M, Wonnenberg B, Herr C, Schneider-Daum N, Lehr C, Bals R, Beisswenger C (2016)
American Journal of Physiology - Lung Cellular and Molecular Physiology, 311 (5): 1015-DOI: 10.1152/ajplung.00158.2016
A 3D co-culture of three human cell lines to model the inflamed intestinal mucosa for safety testing of nanomaterials
Susewind J, Souza Carvalho-Wodarz C, Repnik U, Collnot E, Schneider-Daum N, Griffiths G, Lehr C (2016)
Nanotoxicology, 10 (1): 53-62DOI: 10.3109/17435390.2015.1008065
Invasin-functionalized liposome nanocarriers improve the intracellular delivery of anti-infective drugs
Menina S, Labouta H, Geyer R, Krause T, Gordon S, Dersch P, Lehr C (2016)
RSC Advances, 6 (47): 41622-41629DOI: 10.1039/c6ra02988d
Autologous co-culture of primary human alveolar macrophages and epithelial cells for investigating aerosol medicines. Part II: evaluation of IL-10-loaded microparticles for the treatment of lung inflammation
Hittinger M, Mell N, Huwer H, Loretz B, Schneider-Daum N, Lehr C (2016)
Alternatives to laboratory animals : ATLA, 44 (4): 349-360
Size-Limited Penetration of Nanoparticles into Porcine Respiratory Mucus after Aerosol Deposition
Murgia X, Pawelzyk P, Schaefer U, Wagner C, Willenbacher N, Lehr C (2016)
Biomacromolecules, 17 (4): 1536-1542DOI: 10.1021/acs.biomac.6b00164
Dual flow bioreactor with ultrathin microporous TEER sensing membrane for evaluation of nanoparticle toxicity
Sbrana T, Ucciferri N, Favrè M, Ahmed S, Collnot E, Lehr C, Ahluwalia A, Liley M (2016)
Sensors and Actuators, B: Chemical, 223: 440-446DOI: 10.1016/j.snb.2015.09.078
Impact of PEG and PEG-b-PAGE modified PLGA on nanoparticle formation, protein loading and release
Rietscher R, Czaplewska J, Majdanski T, Gottschaldt M, Schubert U, Schneider M, Lehr C (2016)
International journal of pharmaceutics, 500 (1-2): 187-195DOI: 10.1016/j.ijpharm.2016.01.021
Budesonide Loaded PLGA Nanoparticles for Targeting the Inflamed Intestinal Mucosa--Pharmaceutical Characterization and Fluorescence Imaging
Ali H, Weigmann B, Collnot E, Khan S, Windbergs M, Lehr C (2016)
Pharmaceutical research, 33 (5): 1085-92DOI: 10.1007/s11095-015-1852-6
Nanoparticle-Based Mucosal Vaccines Targeting Tumor-Associated Antigens to Human Dendritic Cells
Brito Baleeiro R, Schweinlin M, Rietscher R, Diedrich A, Czaplewska J, Metzger M, Michael Lehr C, Scherließ R, Hanefeld A, Gottschaldt M, Walden P (2016)
j biomed nanotechnol, 12 (7): 1527-1543DOI: 10.1166/jbn.2016.2267
Autologous co-culture of primary human alveolar macrophages and epithelial cells for investigating aerosol medicines. Part I: Model Characterisation
Hittinger M, Janke J, Huwer H, Scherließ R, Schneider-Daum N, Lehr C (2016)
ATLA Alternatives to Laboratory Animals, 44 (4): 337-347
Preparation of nanosized coacervates of positive and negative starch derivatives intended for pulmonary delivery of proteins
Barthold S, Kletting S, Taffner J, De Souza Carvalho-Wodarz C, Lepeltier E, Loretz B, Lehr C (2016)
Journal of Materials Chemistry B, 4 (13): 2377-2386DOI: 10.1039/c6tb00178e
Sustained-release liquisolid compact tablets containing artemether-lumefantrine as alternate-day regimen for malaria treatment to improve patient compliance
Nnamani P, Ugwu A, Ibezim E, Kenechukwu F, Akpa P, Ogbonna J, Obitte N, Odo A, Windbergs M, Lehr C, Attama A (2016)
International Journal of Nanomedicine, 11: 6365-6378DOI: 10.2147/IJN.S92755
Design of Polyamine-Grafted Starches for Nucleotide Analogue Delivery: In Vitro Evaluation of the Anticancer Activity
Kanber E, Yamada H, Loretz B, Lepeltier E, Lehr C (2016)
Bioconjugate chemistryDOI: 10.1021/acs.bioconjchem.6b00396
Different macro- and micro-rheological properties of native porcine respiratory and intestinal mucus
Bokkasam H, Ernst M, Guenther M, Wagner C, Schaefer U, Lehr C (2016)
International journal of pharmaceutics, 510 (1): 164-167DOI: 10.1016/j.ijpharm.2016.06.035
Human alveolar epithelial cells expressing tight junctions to model the air-blood barrier
Kuehn A, Kletting S, Souza Carvalho-Wodarz C, Repnik U, Griffiths G, Fischer U, Meese E, Huwer H, Wirth D, May T, Schneider-Daum N, Lehr C (2016)
ALTEX, 33 (3): 251-60DOI: 10.14573/altex.1511131
Influence of agglomeration and specific lung lining lipid/protein interaction on short-term inhalation toxicity
Wohlleben W, Driessen M, Raesch S, Schaefer U, Schulze C, Vacano B, Vennemann A, Wiemann M, Ruge C, Platsch H, …, Lehr C, Haase A (2016)
Nanotoxicology, 10 (7): 970-980DOI: 10.3109/17435390.2016.1155671
Self-Assembly and Shape Control of Hybrid Nanocarriers Based on Calcium Carbonate and Carbon Nanodots
Lauth V, Loretz B, Lehr C, Maas M, Rezwan K (2016)
Chemistry of Materials, 28 (11): 3796-3803DOI: 10.1021/acs.chemmater.6b00769
Nanocarriers for optimizing the balance between interfollicular permeation and follicular uptake of topically applied clobetasol to minimize adverse effects
Mathes C, Melero A, Conrad P, Vogt T, Rigo L, Selzer D, Prado W, De Rossi C, Garrigues T, Hansen S, …, Lehr C, Schaefer U (2016)
Journal of Controlled Release, 223: 207-214DOI: 10.1016/j.jconrel.2015.12.010
Antigen delivery via hydrophilic PEG-b-PAGE-b-PLGA nanoparticles boosts vaccination induced T cell immunity
Rietscher R, Schröder M, Janke J, Czaplewska J, Gottschaldt M, Scherließ R, Hanefeld A, Schubert U, Schneider M, Knolle P, Lehr C (2016)
European Journal of Pharmaceutics and Biopharmaceutics, 102: 20-31DOI: 10.1016/j.ejpb.2016.02.014
Tight junctions form a barrier in porcine hair follicles
Mathes C, Brandner J, Laue M, Raesch S, Hansen S, Failla A, Vidal S, Moll I, Schaefer U, Lehr C (2016)
European Journal of Cell Biology, 95 (2): 89-99DOI: 10.1016/j.ejcb.2015.12.001
The effect of polymer size and charge of molecules on permeation through synovial membrane and accumulation in hyaline articular cartilage
Sterner B, Harms M, Wöll S, Weigandt M, Windbergs M, Lehr C (2016)
European Journal of Pharmaceutics and Biopharmaceutics, 101: 126-136DOI: 10.1016/j.ejpb.2016.02.004
Label-free analysis of drug delivery systems and cellular interaction studies using confocal Raman microscopy
Kann B (2016)
Book
Tunable nanoparticles for the local delivery of protein drugs for improved vaccination
Rietscher R (2016)
Book
Immortalization of human alveolar epithelial cells
Kühn A (2016)
Book
Mathematical characterization of skin absorption
Selzer D (2016)
Book
Drug delivery to the hair follicle
Mathes C (2016)
Book
A new cell line-based coculture model of the human air-blood barrier to evaluate the interaction with aerosolized drug carriers
Kletting S (2016)
Book
The nanoparticle corona in the deep lung
Räsch S (2016)
Book
Confocal Raman microscopy in skin drug delivery research
Franzen L (2016)
Book
2015
Overview of Inhaled Nanopharmaceuticals
Barthold S, Kunschke N, Murgia X, Loretz B, Carvalho Wodarz C, Lehr C (2015)
Book
Not just for tumor targeting: Unmet medical needs and opportunities for nanomedicine
Lepeltier E, Nuhn L, Lehr C, Zentel R (2015)
Nanomedicine, 10 (20): 3147-3166DOI: 10.2217/nnm.15.132
Preclinical safety and efficacy models for pulmonary drug delivery of antimicrobials with focus on in vitro models
Hittinger M, Juntke J, Kletting S, Schneider-Daum N, de Souza Carvalho C, Lehr C (2015)
Advanced Drug Delivery Reviews, 85: 44-56DOI: 10.1016/j.addr.2014.10.011
Cyclodextrin-based star polymers as a versatile platform for nanochemotherapeutics: Enhanced entrapment and uptake of idarubicin
Nafee N, Hirosue M, Loretz B, Wenz G, Lehr C (2015)
Colloids and surfaces. B, Biointerfaces, 129: 30-8DOI: 10.1016/j.colsurfb.2015.03.014
Spatial separation of the processing and MHC class I loading compartments for cross-presentation of the tumor-associated antigen HER2/neu by human dendritic cells
Baleeiro R, Rietscher R, Diedrich A, Czaplewska J, Lehr C, Scherließ R, Hanefeld A, Gottschaldt M, Walden P (2015)
Oncoimmunology, 4 (11)DOI: 10.1080/2162402X.2015.1047585
Proteomic and Lipidomic Analysis of Nanoparticle Corona upon Contact with Lung Surfactant Reveals Differences in Protein, but Not Lipid Composition
Raesch S, Tenzer S, Storck W, Rurainski A, Selzer D, Ruge C, Perez-Gil J, Schaefer U, Lehr C (2015)
ACS Nano, 9 (12): 11872-11885DOI: 10.1021/acsnano.5b04215
Mono- and Cocultures of Bronchial and Alveolar Epithelial Cells Respond Differently to Proinflammatory Stimuli and Their Modulation by Salbutamol and Budesonide
Haghi M, Hittinger M, Zeng Q, Oliver B, Traini D, Young P, Huwer H, Schneider-Daum N, Lehr C (2015)
Molecular pharmaceutics, 12 (8): 2625-32DOI: 10.1021/acs.molpharmaceut.5b00124
Bacteriomimetic invasin-functionalized nanocarriers for intracellular delivery
Labouta H, Menina S, Kochut A, Gordon S, Geyer R, Dersch P, Lehr C (2015)
Journal of Controlled Release, 220: 414-424DOI: 10.1016/j.jconrel.2015.10.052
Macrophage uptake of cylindrical microparticles investigated with correlative microscopy
Tscheka C, Hittinger M, Lehr C, Schneider-Daum N, Schneider M (2015)
European Journal of Pharmaceutics and Biopharmaceutics, 95: 151-155DOI: 10.1016/j.ejpb.2015.03.010
Transfection system of amino-functionalized calcium phosphate nanoparticles: in vitro efficacy, biodegradability, and immunogenicity study
Mostaghaci B, Susewind J, Kickelbick G, Lehr C, Loretz B (2015)
ACS applied materials & interfaces, 7 (9): 5124-33DOI: 10.1021/am507193a
In vitro–in vivo correlation
Barakat A, Krämer J, Souza Carvalho C, Lehr C (2015)
Dissolution Technologies, 22 (2): 19-22DOI: 10.14227/DT220215P19
A design of experiment study of nanoprecipitation and nano spray drying as processes to prepare plga nano-and microparticles with defined sizes and size distributions
Draheim C, De Crécy F, Hansen S, Collnot E, Lehr C (2015)
Pharmaceutical research, 32 (8): 2609-2624DOI: 10.1007/s11095-015-1647-9
Enhanced uptake and siRNA-mediated knockdown of a biologically relevant gene using cyclodextrin polyrotaxane
Dandekar P, Jain R, Keil M, Loretz B, Koch M, Wenz G, Lehr C (2015)
Journal of Materials Chemistry B, 3 (13): 2590-2598DOI: 10.1039/c4tb01821d
Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro
Schwagerus E, Sladek S, Buckley S, Armas-Capote N, La Rosa D, Harvey B, Fischer H, Illek B, Huwer H, Schneider-Daum N, Lehr C, Ehrhardt C (2015)
Pflugers Archiv European Journal of Physiology, 467 (11): 2257-2273DOI: 10.1007/s00424-015-1693-5
P-glycoprotein interactions of novel psychoactive substances - stimulation of ATP consumption and transport across Caco-2 monolayers
Meyer M, Wagmann L, Schneider-Daum N, Loretz B, Souza Carvalho C, Lehr C, Maurer H (2015)
Biochemical pharmacology, 94 (3): 220-6DOI: 10.1016/j.bcp.2015.01.008
Efficient nanoparticle-mediated needle-free transcutaneous vaccination via hair follicles requires adjuvantation
Mittal A, Schulze K, Ebensen T, Weißmann S, Hansen S, Lehr C, Guzmán C (2015)
Nanomedicine: Nanotechnology, Biology, and Medicine, 11 (1): 147-154DOI: 10.1016/j.nano.2014.08.009
Semi-automated nanoprecipitation-system - An option for operator independent, scalable and size adjustable nanoparticle synthesis
Rietscher R, Thum C, Lehr C, Schneider M (2015)
Pharmaceutical research, 32 (6): 1859-1863DOI: 10.1007/s11095-014-1612-z
Organic cation transporter function in different in vitro models of human lung epithelium
Salomon J, Gausterer J, Yahara T, Hosoya K, Huwer H, Hittinger M, Schneider-Daum N, Lehr C, Ehrhardt C (2015)
European Journal of Pharmaceutical Sciences, 80: 82-88DOI: 10.1016/j.ejps.2015.08.007
In vivo genome editing using nuclease-encoding mRNA corrects SP-B deficiency
Mahiny A, Dewerth A, Mays L, Alkhaled M, Mothes B, Malaeksefat E, Loretz B, Rottenberger J, Brosch D, Reautschnig P, …, Lehr C, Kormann M (2015)
Nature biotechnology, 33 (6): 584-6DOI: 10.1038/nbt.3241
The buccal mucosa as a route for TiO2 nanoparticle uptake
Teubl B, Leitinger G, Schneider M, Lehr C, Fröhlich E, Zimmer A, Roblegg E (2015)
Nanotoxicology, 9 (2): 253-261DOI: 10.3109/17435390.2014.921343
Inverse micellar sugar glass (IMSG) nanoparticles for transfollicular vaccination
Mittal A, Schulze K, Ebensen T, Weissmann S, Hansen S, Guzmán C, Lehr C (2015)
Journal of Controlled Release, 206: 140-152DOI: 10.1016/j.jconrel.2015.03.017
Squalenoylation of Chitosan: A Platform for Drug Delivery?
Lepeltier E, Loretz B, Desmaele D, Zapp J, Herrmann J, Couvreur P, Lehr C (2015)
Biomacromolecules, 16 (9): 2930-9DOI: 10.1021/acs.biomac.5b00840
Miniature in vitro dissolution testing of powders for inhalation
May S, Kind S, Jensen B, Wolkenhauer M, Schneider M, Lehr C (2015)
Dissolution Technologies, 22 (3): 40-51DOI: 10.14227/DT220315P40
Solid Phase Extraction as an Innovative Separation Method for Measuring Free and Entrapped Drug in Lipid Nanoparticles
Guillot A, Couffin A, Sejean X, Navarro F, Limberger M, Lehr C (2015)
Pharmaceutical research, 32 (12): 3999-4009DOI: 10.1007/s11095-015-1761-8
Focused Ultrasound as a Scalable and Contact-Free Method to Manufacture Protein-Loaded PLGA Nanoparticles
Schiller S, Hanefeld A, Schneider M, Lehr C (2015)
Pharmaceutical research, 32 (9): 2995-3006DOI: 10.1007/s11095-015-1681-7
Dimethylaminoethyl methacrylate copolymer-siRNA nanoparticles for silencing a therapeutically relevant gene in macrophages
Jain R, Dandekar P, Loretz B, Koch M, Lehr C (2015)
MedChemComm, 6 (4): 691-701DOI: 10.1039/c4md00490f
A strategy for in-silico prediction of skin absorption in man
Selzer D, Neumann D, Neumann H, Kostka K, Lehr C, Schaefer U (2015)
European Journal of Pharmaceutics and Biopharmaceutics, 95: 68-76DOI: 10.1016/j.ejpb.2015.05.002
Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology
Gordon S, Daneshian M, Bouwstra J, Caloni F, Constant S, Davies D, Dandekar G, Guzman C, Fabian E, Haltner E, …, Walles H, Lehr C (2015)
ALTEX, 32 (4): 327-378DOI: 10.14573/altex.1510051
Inhalable Clarithromycin Microparticles for Treatment of Respiratory Infections
Dimer F, De Souza Carvalho-Wodarz C, Haupenthal J, Hartmann R, Lehr C (2015)
Pharmaceutical research, 32 (12): 3850-3861DOI: 10.1007/s11095-015-1745-8
Advanced formulations of cyclosporine A and budesonide for the therapy of inflammatory bowel disease
Draheim C (2015)
Book
Synthesis and Characterization of Novel Amino-modified Calcium Phosphate Nanoparticles Used for Gene Delivery Applications
Mostaghaci B (2015)
Development and characterization of nanoparticular systems for an innovating therapeutic concept for targeting Inflammatory Bowel Diseases using the Quality-by-Design approach
Guillot A (2015)
2014
Transfollicular delivery takes root
Hansen S, Lehr C (2014)
Expert Review of Vaccines, 13 (1): 5-7DOI: 10.1586/14760584.2014.862500
Overcoming the pulmonary barrier: New insights to improve the efficiency of inhaled therapeutics
Murgia X, de Souza Carvalho C, Lehr C (2014)
European Journal of Nanomedicine, 6 (3): 157-169DOI: 10.1515/ejnm-2014-0019
Carrier interactions with the biological barriers of the lung: Advanced in vitro models and challenges for pulmonary drug delivery
de Souza Carvalho C, Daum N, Lehr C (2014)
Advanced Drug Delivery Reviews, 75: 129-140DOI: 10.1016/j.addr.2014.05.014
Budesonide loaded nanoparticles with pH-sensitive coating for improved mucosal targeting in mouse models of inflammatory bowel diseases
Ali H, Weigmann B, Neurath M, Collnot E, Windbergs M, Lehr C (2014)
Journal of Controlled Release, 183 (1): 167-177DOI: 10.1016/j.jconrel.2014.03.039
Crystal suspensions of poorly soluble peptides for intra-articular application: A novel approach for biorelevant assessment of their in vitro release
Sterner B, Harms M, Weigandt M, Windbergs M, Lehr C (2014)
International journal of pharmaceutics, 461 (1-2): 46-53DOI: 10.1016/j.ijpharm.2013.11.031
Design of starch-graft-PEI polymers: an effective and biodegradable gene delivery platform
Yamada H, Loretz B, Lehr C (2014)
Biomacromolecules, 15 (5): 1753-61DOI: 10.1021/bm500128k
Nanoencapsulation in lipid-core nanocapsules controls mometasone furoate skin permeability rate and its penetration to the deeper skin layers
Melero A, Ferreira Ourique A, Stanisçuaski Guterres S, Raffin Pohlmann A, Lehr C, Ruver Beck R, Schaefer U (2014)
Skin Pharmacology and Physiology, 27 (4): 217-228DOI: 10.1159/000354921
Non-invasive determination of cellular oxygen consumption as novel cytotoxicity assay for nanomaterials
Neumeyer A, Bukowski M, Veith M, Lehr C, Daum N (2014)
Nanotoxicology, 8 (1): 50-60DOI: 10.3109/17435390.2012.747639
Quantification of nanoparticle uptake into hair follicles in pig ear and human forearm
Raber A, Mittal A, Schäfer J, Bakowsky U, Reichrath J, Vogt T, Schaefer U, Hansen S, Lehr C (2014)
Journal of Controlled Release, 179 (1): 25-32DOI: 10.1016/j.jconrel.2014.01.018
Development of artemether-loaded nanostructured lipid carrier (NLC) formulation for topical application
Nnamani P, Hansen S, Windbergs M, Lehr C (2014)
International journal of pharmaceutics, 477 (1-2): 208-217DOI: 10.1016/j.ijpharm.2014.10.004
Characterization and evaluation of a modified PVPA barrier in comparison to Caco-2 cell monolayers for combined dissolution and permeation testing
Gantzsch S, Kann B, Ofer-Glaessgen M, Loos P, Berchtold H, Balbach S, Eichinger T, Lehr C, Schaefer U, Windbergs M (2014)
Journal of Controlled Release, 175 (1): 79-86DOI: 10.1016/j.jconrel.2013.12.009
In vitro toxicological screening of nanoparticles on primary human endothelial cells and the role of flow in modulating cell response
Ucciferri N, Collnot E, Gaiser B, Tirella A, Stone V, Domenici C, Lehr C, Ahluwalia A (2014)
Nanotoxicology, 8 (6): 697-708DOI: 10.3109/17435390.2013.831500
The cell line NCl-H441 is a useful in vitro model for transport studies of human distal lung epithelial barrier
Salomon J, Muchitsch V, Gausterer J, Schwagerus E, Huwer H, Daum N, Lehr C, Ehrhardt C (2014)
Molecular pharmaceutics, 11 (3): 995-1006DOI: 10.1021/mp4006535
Antibiotic-free nanotherapeutics: Ultra-small, mucus-penetrating solid lipid nanoparticles enhance the pulmonary delivery and anti-virulence efficacy of novel quorum sensing inhibitors
Nafee N, Husari A, Maurer C, Lu C, De Rossi C, Steinbach A, Hartmann R, Lehr C, Schneider M (2014)
Journal of Controlled Release, 192: 131-140DOI: 10.1016/j.jconrel.2014.06.055
Statistical comparison of dissolution profiles to predict the bioequivalence of extended release formulations
Gomez-Mantilla J, Schaefer U, Casabo V, Lehr T, Lehr C (2014)
AAPS Journal, 16 (4): 791-801DOI: 10.1208/s12248-014-9615-6
Dissolution testing of powders for inhalation
May S, Jensen B, Weiler C, Wolkenhauer M, Schneider M, Lehr C (2014)
Pharmaceutical research, 31 (11): 3211-3224DOI: 10.1007/s11095-014-1413-4
Polyester-idarubicin nanoparticles and a polymer-photosensitizer complex as potential drug formulations for cell-mediated drug delivery
Blaudszun A, Lian Q, Schnabel M, Loretz B, Steinfeld U, Lee H, Wenz G, Lehr C, Schneider M, Philippi A (2014)
International journal of pharmaceutics, 474 (1-2): 70-9DOI: 10.1016/j.ijpharm.2014.07.048
Exploring the follicular route for transcutaneous vaccination using nanoparticles
Mittal A (2014)
Statistical approaches to perform dissolution profile comparisons
Gómez Mantilla J (2014)
Book
Starch based cationic polymers for gene delivery
Yamada H (2014)
Book
Endothelial and epithelial differentiation potential of human adult stem cells PSC and MSC and the relation of ZO-1 isoform ratio to epithelial differentiation in Caco-2
Meier K (2014)
Book
2013
Particle based vaccine formulations for transcutaneous immunization
Mittal A, Raber A, Lehr C, Hansen S (2013)
Human Vaccines and Immunotherapeutics, 9 (9): 1950-1955DOI: 10.4161/hv.25217
Improved input parameters for diffusion models of skin absorption
Hansen S, Lehr C, Schaefer U (2013)
Advanced Drug Delivery Reviews, 65 (2): 251-264DOI: 10.1016/j.addr.2012.04.011
Modeling the human skin barrier-Towards a better understanding of dermal absorption
Hansen S, Lehr C, Schaefer U (2013)
Advanced Drug Delivery Reviews, 65 (2): 149-151DOI: 10.1016/j.addr.2012.12.002
Pulmonary drug delivery: From generating aerosols to overcoming biological barriers-therapeutic possibilities and technological challenges
Ruge C, Kirch J, Lehr C (2013)
The Lancet Respiratory Medicine, 1 (5): 402-413DOI: 10.1016/S2213-2600(13)70072-9
Mucus as a barrier to lipophilic drugs
Sigurdsson H, Kirch J, Lehr C (2013)
International journal of pharmaceutics, 453 (1): 56-64DOI: 10.1016/j.ijpharm.2013.05.040
Crossing biological barriers for advanced drug delivery
Schneider M, Windbergs M, Daum N, Loretz B, Collnot E, Hansen S, Schaefer U, Lehr C (2013)
European Journal of Pharmaceutics and Biopharmaceutics, 84 (2): 239-241DOI: 10.1016/j.ejpb.2013.03.009
Nanomedicines for the treatment of inflammatory bowel diseases
Ali H, Collnot E, Windbergs M, Lehr C (2013)
European Journal of Nanomedicine, 5 (1)DOI: 10.1515/ejnm-2013-0004
From the structure of the skin barrier and dermal formulations to in vitro transport models for skin absorption
Windbergs M, Hansen S, Schroeter A, Schaefer U, Lehr C, Bouwstra J (2013)
Skin Pharmacology and Physiology, 26 (4-6): 317-330DOI: 10.1159/000351936
PEG-functionalized microparticles selectively target inflamed mucosa in inflammatory bowel disease
Lautenschläger C, Schmidt C, Lehr C, Fischer D, Stallmach A (2013)
European Journal of Pharmaceutics and Biopharmaceutics, 85 (3 PART A): 578-586DOI: 10.1016/j.ejpb.2013.09.016
Biopharmaceutical in vitro characterization of CPZEN-45, a drug candidate for inhalation therapy of tuberculosis
Salomon J, Galeron P, Schulte N, Morow P, Severynse-Stevens D, Huwer H, Daum N, Lehr C, Hickey A, Ehrhardt C (2013)
Therapeutic delivery, 4 (8): 915-923DOI: 10.4155/tde.13.62
In situ drug release monitoring with a fiber-optic system: Overcoming matrix interferences using derivative spectrophotometry
Guillot A, Limberge M, Krämer J, Lehr C (2013)
Dissolution Technologies, 20 (2): 15-19
One-step synthesis of nanosized and stable amino-functionalized calcium phosphate particles for DNA transfection
Mostaghaci B, Loretz B, Haberkorn R, Kickelbick G, Lehr C (2013)
Chemistry of Materials, 25 (18): 3667-3674DOI: 10.1021/cm401886u
Nanoparticle geometry and surface orientation influence mode of cellular uptake
Herd H, Daum N, Jones A, Huwer H, Ghandehari H, Lehr C (2013)
ACS Nano, 7 (3): 1961-1973DOI: 10.1021/nn304439f
Non-invasive delivery of nanoparticles to hair follicles: A perspective for transcutaneous immunization
Mittal A, Raber A, Schaefer U, Weissmann S, Ebensen T, Schulze K, Guzmán C, Lehr C, Hansen S (2013)
Vaccine, 31 (34): 3442-3451DOI: 10.1016/j.vaccine.2012.12.048
Nano- and microscaled particles for drug targeting to inflamed intestinal mucosa - A first in vivo study in human patients
Schmidt C, Lautenschlaeger C, Collnot E, Schumann M, Bojarski C, Schulzke J, Lehr C, Stallmach A (2013)
Journal of Controlled Release, 165 (2): 139-145DOI: 10.1016/j.jconrel.2012.10.019
Finite dose skin mass balance including the lateral part: Comparison between experiment, pharmacokinetic modeling and diffusion models
Selzer D, Hahn T, Naegel A, Heisig M, Kostka K, Lehr C, Neumann D, Schaefer U, Wittum G (2013)
Journal of Controlled Release, 165 (2): 119-128DOI: 10.1016/j.jconrel.2012.10.009
Pulmonary surfactant is indispensable in order to simulate the in vivo situation
Schleh C, Kreyling W, Lehr C (2013)
Particle and Fibre Toxicology, 10 (1)DOI: 10.1186/1743-8977-10-6
Permutation Test (PT) and Tolerated Difference Test (TDT)
Gómez-Mantilla J, Casabó V, Schaefer U, Lehr C (2013)
International journal of pharmaceutics, 441 (1-2): 458-467DOI: 10.1016/j.ijpharm.2012.11.008
Budesonide Loaded pH-sensitive PLGA Nanoparticles for the Treatment of Inflammatory Bowel Disease
Ali H (2013)
Novel cell based in vitro models to study nanoparticle interaction with the inflamed intestinal mucosa
Leonard F (2013)
Book
Dissolution testing of powders for inhalation
May S (2013)
Book
Implementation of a lipid-coated membrane in a combined dissolution and permeation system and comparison to Caco-2 cell monolayers
Gantzsch S (2013)
Book
2012
Isolation, cultivation, and application of human alveolar epithelial cells
Daum N, Kuehn A, Hein S, Schaefer U, Huwer H, Lehr C (2012)
Methods in molecular biology (Clifton, N.J.), 806: 31-42DOI: 10.1007/978-1-61779-367-7_3
Optical tweezers reveal relationship between microstructure and nanoparticle penetration of pulmonary mucus
Kirch J, Schneider A, Abou B, Hopf A, Schaefer U, Schneider M, Schall C, Wagner C, Lehr C (2012)
Proceedings of the National Academy of Sciences of the United States of America, 109 (45): 18355-18360DOI: 10.1073/pnas.1214066109
Nanoparticles for transcutaneous vaccination
Hansen S, Lehr C (2012)
Microbial Biotechnology, 5 (2): 156-167DOI: 10.1111/j.1751-7915.2011.00284.x
Nano- and microparticulate drug carriers for targeting of the inflamed intestinal mucosa
Collnot E, Ali H, Lehr C (2012)
Journal of Controlled Release, 161 (2): 235-246DOI: 10.1016/j.jconrel.2012.01.028
Characterization and evaluation of β-glucan formulations as injectable implants for protein and peptide delivery
Jacobs S, Bunt C, Wu Z, Lehr C, Rupenthal I (2012)
Drug Development and Industrial Pharmacy, 38 (11): 1337-1343DOI: 10.3109/03639045.2011.650646
Process optimization and biocompatibility of cell carriers suitable for automated magnetic manipulation
Krejci I, Piana C, Howitz S, Wegener T, Fiedler S, Zwanzig M, Schmitt D, Daum N, Meier K, Lehr C, …, Wirth M, Gabor F (2012)
Acta Biomaterialia, 8 (3): 1239-1247DOI: 10.1016/j.actbio.2011.08.031
A hydrophobic starch polymer for nanoparticle-mediated delivery of docetaxel
Dandekar P, Jain R, Stauner T, Loretz B, Koch M, Wenz G, Lehr C (2012)
Macromolecular Bioscience, 12 (2): 184-94DOI: 10.1002/mabi.201100244
Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes
Dandekar P, Jain R, Keil M, Loretz B, Muijs L, Schneider M, Auerbach D, Jung G, Lehr C, Wenz G (2012)
Journal of controlled release : official journal of the Controlled Release Society, 164 (3): 387-93DOI: 10.1016/j.jconrel.2012.06.040
Treatment of lung cancer via telomerase inhibition
Nafee N, Schneider M, Friebel K, Dong M, Schaefer U, Mürdter T, Lehr C (2012)
European Journal of Pharmaceutics and Biopharmaceutics, 80 (3): 478-489DOI: 10.1016/j.ejpb.2011.11.019
Atomic force microscopy and analytical ultracentrifugation for probing nanomaterial protein interactions
Schaefer J, Schulze C, Marxer E, Schaefer U, Wohlleben W, Bakowsky U, Lehr C (2012)
ACS Nano, 6 (6): 4603-4614DOI: 10.1021/nn202657q
Influence of the application area on finite dose permeation in relation to drug type applied
Hahn T, Selzer D, Neumann D, Kostka K, Lehr C, Schaefer U (2012)
Experimental Dermatology, 21 (3): 233-235DOI: 10.1111/j.1600-0625.2011.01424.x
Mucociliary clearance of micro- and nanoparticles is independent of size, shape and charge-an ex vivo and in silico approach
Kirch J, Guenther M, Doshi N, Schaefer U, Schneider M, Mitragotri S, Lehr C (2012)
Journal of Controlled Release, 159 (1): 128-134DOI: 10.1016/j.jconrel.2011.12.015
Pulmonary delivery and tissue distribution of aerosolized antisense 2'-O-Methyl RNA containing nanoplexes in the isolated perfused and ventilated rat lung
Dong M, Murdter T, Philippi C, Loretz B, Schaefer U, Lehr C, Schwab M, Ammon-Treiber S (2012)
European Journal of Pharmaceutics and Biopharmaceutics, 81 (3): 478-85DOI: 10.1016/j.ejpb.2012.04.022
Screening of budesonide nanoformulations for treatment of inflammatory bowel disease in an inflamed 3D cell-culture model
Leonard F, Ali H, Collnot E, Crielaard B, Lammers T, Storm G, Lehr C (2012)
ALTEX, 29 (3): 275-285
Soluplus® as an effective absorption enhancer of poorly soluble drugs in vitro and in vivo
Linn M, Collnot E, Djuric D, Hempel K, Fabian E, Kolter K, Lehr C (2012)
European Journal of Pharmaceutical Sciences, 45 (3): 336-343DOI: 10.1016/j.ejps.2011.11.025
The interplay of lung surfactant proteins and lipids assimilates the macrophage clearance of nanoparticles
Ruge C, Schaefer U, Herrmann J, Kirch J, Cañadas O, Echaide M, Pérez-Gil J, Casals C, Müller R, Lehr C (2012)
PLoS ONE, 7 (7)DOI: 10.1371/journal.pone.0040775
Dissolution techniques for in vitro testing of dry powders for inhalation
May S, Jensen B, Wolkenhauer M, Schneider M, Lehr C (2012)
Pharmaceutical research, 29 (8): 2157-2166DOI: 10.1007/s11095-012-0744-2
Bio-nano interactions in the peripheral lungs
Ruge C (2012)
Book
In vitro characterization of the novel solubility enhancing excipient Soluplus®
Linn M (2012)
Book
The role of fluid dynamics, microstructure and mucociliary clearance in the micro- and macroscopic barrier properties of pulmonary mucus
Kirch J (2012)
Book
Evaluation of finite dose skin absorption experiments with respect to experimental setup and mathematical modelling
Hahn T (2012)
Book
2011
Uptake of nanoparticles by alveolar macrophages is triggered by surfactant protein A
Ruge C, Kirch J, Cañadas O, Schneider M, Perez-Gil J, Schaefer U, Casals C, Lehr C (2011)
Nanomedicine: Nanotechnology, Biology, and Medicine, 7 (6): 690-693DOI: 10.1016/j.nano.2011.07.009
A new Pharmaceutical Aerosol Deposition Device on Cell Cultures (PADDOCC) to evaluate pulmonary drug absorption for metered dose dry powder formulations
Hein S, Bur M, Schaefer U, Lehr C (2011)
European Journal of Pharmaceutics and Biopharmaceutics, 77 (1): 132-138DOI: 10.1016/j.ejpb.2010.10.003
Propidium iodide labeling of nanoparticles as a novel tool for the quantification of cellular binding and uptake
Neumeyer A, Bukowski M, Veith M, Lehr C, Daum N (2011)
Nanomedicine: Nanotechnology, Biology, and Medicine, 7 (4): 410-419DOI: 10.1016/j.nano.2010.12.007
Interaction of metal oxide nanoparticles with lung surfactant protein A
Schulze C, Schaefer U, Ruge C, Wohlleben W, Lehr C (2011)
European Journal of Pharmaceutics and Biopharmaceutics, 77 (3): 376-383DOI: 10.1016/j.ejpb.2010.10.013
Tissue slice model of human lung cancer to investigate telomerase inhibition by nanoparticle delivery of antisense 2'-O-methyl-RNA
Dong M, Philippi C, Loretz B, Nafee N, Schaefer U, Friedel G, Ammon-Treiber S, Griese E, Lehr C, Klotz U, Murdter T (2011)
International journal of pharmaceutics, 419 (1-2): 33-42DOI: 10.1016/j.ijpharm.2011.07.009
Improved photostability and reduced skin permeation of tretinoin
Ourique A, Melero A, Silva C, Schaefer U, Pohlmann A, Guterres S, Lehr C, Kostka K, Beck R (2011)
European Journal of Pharmaceutics and Biopharmaceutics, 79 (1): 95-101DOI: 10.1016/j.ejpb.2011.03.008
Transport of Metal Oxide Nanoparticles Across Calu-3 Cell Monolayers Modelling the Air-Blood Barrier
Schulze C, Schaefer U, Voetz M, Wohlleben W, Venzago C, Lehr C (2011)
EURO-NanoTox-Letters, 3 (1): 1-10DOI: 10.1515/entl-2015-0003
Template-assisted polyelectrolyte encapsulation of nanoparticles into dispersible, hierarchically nanostructured microfibers
Kohler D, Schneider M, Krüger M, Lehr C, Möhwald H, Wang D (2011)
Advanced Materials, 23 (11): 1376-1379DOI: 10.1002/adma.201004048
Computational fluid dynamics of nanoparticle disposition in the airways
Kirch J, Guenther M, Schaefer U, Schneider M, Lehr C (2011)
Computing and Visualization in Science, 14 (7): 301-308DOI: 10.1007/s00791-012-0184-x
An in vitro triple cell co-culture model with primary cells mimicking the human alveolar epithelial barrier
Lehmann A, Daum N, Bur M, Lehr C, Gehr P, Rothen-Rutishauser B (2011)
European Journal of Pharmaceutics and Biopharmaceutics, 77 (3): 398-406DOI: 10.1016/j.ejpb.2010.10.014
Enhanced cellular delivery of idarubicin by surface modification of propyl starch nanoparticles employing pteroic acid conjugated polyvinyl alcohol
Jain R, Dandekar P, Loretz B, Melero A, Stauner T, Wenz G, Koch M, Lehr C (2011)
International journal of pharmaceutics, 420 (1): 147-55DOI: 10.1016/j.ijpharm.2011.08.030
Finite dose skin penetration
Naegel A, Hahn T, Schaefer U, Lehr C, Heisig M, Wittum G (2011)
Computing and Visualization in Science, 14 (7): 327-339DOI: 10.1007/s00791-012-0186-8
Selective follicular targeting by modification of the particle sizes
Patzelt A, Richter H, Knorr F, Schäfer U, Lehr C, Dähne L, Sterry W, Lademann J (2011)
Journal of Controlled Release, 150 (1): 45-48DOI: 10.1016/j.jconrel.2010.11.015
Asymmetric particles for pulmonary drug delivery
Kohler D (2011)
Book
Multilayer coating of gold nanoparticles (AuNP) with drug-polymer complex
Reum N (2011)
Book
New models for telomerase inhibition by antisense 2'-O-methyl-RNA in lung cancer therapy
Dong M (2011)
Book
2010
Pulmonary drug delivery
Henning A, Hein S, Schneider M, Bur M, Lehr C (2010)
Book, 197DOI: 10.1007/978-3-642-00477-3_6
Telomerase as an emerging target to fight cancer - Opportunities and challenges for nanomedicine
Philippi C, Loretz B, Schaefer U, Lehr C (2010)
Journal of Controlled Release, 146 (2): 228-240DOI: 10.1016/j.jconrel.2010.03.025
Preparation of elongated polyelectrolyte particles from particles
Kohler D, Schneider M, Lehr C, Krüger M, Möhwald H, Wang D (2010)
European Cells and Materials, 20 (SUPPL.3)
Plant genetic factors for iron homeostasis affect iron bioavailability in Caco-2 cells
Maurer F, Daum N, Schaefer U, Lehr C, Bauer P (2010)
Food Research International, 43 (6): 1661-1665DOI: 10.1016/j.foodres.2010.05.008
Infrared densitometry
Hahn T, Hansen S, Neumann D, Kostka K, Lehr C, Muys L, Schaefer U (2010)
Skin Pharmacology and Physiology, 23 (4): 183-192DOI: 10.1159/000288165
Cellular responses after exposure of lung cell cultures to secondary organic aerosol particles
Gaschen A, Lang D, Kalberer M, Savi M, Geiser T, Gazdhar A, Lehr C, Bur M, Dommen J, Baltensperger U, Geiser M (2010)
Environmental Science and Technology, 44 (4): 1424-1430DOI: 10.1021/es902261m
Drug transport across pulmonary epithelial cell monolayers: effects of particle size, apical liquid volume, and deposition technique
Bur M, Huwer H, Muys L, Lehr C (2010)
Journal of aerosol medicine and pulmonary drug delivery, 23 (3): 119-27DOI: 10.1089/jamp.2009.0757
Vitamin e TPGS P-glycoprotein inhibition mechanism
Collnot E, Baldes C, Schaefer U, Edgar K, Wempe M, Lehr C (2010)
Molecular pharmaceutics, 7 (3): 642-651DOI: 10.1021/mp900191s
Online monitoring of transepithelial electrical resistance (TEER) in an apparatus for combined dissolution and permeation testing
Muendoerfer M, Schaefer U, Koenig P, Walk J, Loos P, Balbach S, Eichinger T, Lehr C (2010)
International journal of pharmaceutics, 392 (1-2): 134-140DOI: 10.1016/j.ijpharm.2010.03.046
Wistar rat skin as surrogate for human skin in nortriptyline hydrochloride patch studies
Melero A, Lehr C, Schäfer U, Garrigues T (2010)
International journal of pharmaceutics, 384 (1-2): 137-139DOI: 10.1016/j.ijpharm.2009.09.028
Multilayer coating of gold nanoparticles with drug-polymer coadsorbates
Reum N, Fink-Straube C, Klein T, Hartmann R, Lehr C, Schneider M (2010)
Langmuir, 26 (22): 16901-16908DOI: 10.1021/la103109b
Nanoparticles made from novel starch derivatives for transdermal drug delivery
Santander-Ortega M, Stauner T, Loretz B, Ortega-Vinuesa J, Bastos-Gonzalez D, Wenz G, Schaefer U, Lehr C (2010)
Journal of controlled release : official journal of the Controlled Release Society, 141 (1): 85-92DOI: 10.1016/j.jconrel.2009.08.012
In vitro and in vivo performance of biocompatible negatively-charged salbutamol-loaded nanoparticles
Rytting E, Bur M, Cartier R, Bouyssou T, Wang X, Krüger M, Lehr C, Kissel T (2010)
Journal of Controlled Release, 141 (1): 101-107DOI: 10.1016/j.jconrel.2009.08.021
Differential cell reaction upon Toll-like receptor 4 and 9 activation in human alveolar and lung interstitial macrophages
Hoppstädter J, Diesel B, Zarbock R, Breinig T, Monz D, Koch M, Meyerhans A, Gortner L, Lehr C, Huwer H, Kiemer A (2010)
Respiratory Research, 11DOI: 10.1186/1465-9921-11-124
A three-dimensional coculture of enterocytes, monocytes and dendritic cells to model inflamed intestinal mucosa in vitro
Leonard F, Collnot E, Lehr C (2010)
Molecular pharmaceutics, 7 (6): 2103-2119DOI: 10.1021/mp1000795
A dynamic ratio of the α+ and α- isoforms of the tight junction protein ZO-1 is characteristic of Caco-2 cells and correlates with their degree of differentiation
Ciana A, Meier K, Daum N, Gerbes S, Veith M, Lehr C, Minetti G (2010)
Cell Biology International, 34 (6): 669-678DOI: 10.1042/CBI20090067
The Pharmaceutical Aerosol Deposition Device on Cell Cultures (PADDOCC) in vitro system
Hein S, Bur M, Kolb T, Muellinger B, Schaefer U, Lehr C (2010)
ATLA Alternatives to Laboratory Animals, 38 (4): 285-295
Nanoparticle mediated delivery of 2′-O-methyl-RNA leads to efficient telomerase inhibition and telomere shortening in human lung cancer cells
Beisner J, Dong M, Taetz S, Nafee N, Griese E, Schaefer U, Lehr C, Klotz U, Mürdter T (2010)
Lung Cancer, 68 (3): 346-354DOI: 10.1016/j.lungcan.2009.07.010
Influence of particle size and material properties on mucociliary clearance from the airways
Henning A, Schneider M, Nafee N, Muijs L, Rytting E, Wang X, Kissel T, Grafahrend D, Klee D, Lehr C (2010)
Journal of aerosol medicine and pulmonary drug delivery, 23 (4): 233-241DOI: 10.1089/jamp.2009.0806
Calorimetric and spectrophotometric investigation of PLGA nanoparticles and their complex with DNA
Khvedelidze M, Mdzinarashvili T, Partskhaladze T, Nafee N, Schaefer U, Lehr C, Schneider M (2010)
Journal of Thermal Analysis and Calorimetry, 99 (1): 337-348DOI: 10.1007/s10973-009-0137-x
Combined assessment of drug dissolution and epithelial permeability
Mündörfer M (2010)
Book
Pharmaceutical aerosol deposition device on cell cultures (PADDOCC)
Hein S (2010)
Book
The potential of silica nanoparticles as oral drug carriers
Neumeyer A (2010)
Book
Transport of metal oxide nanoparticles across the human air-blood barrier
Schulze C (2010)
Book
2007
Mechanism of inhibition of P-glycoprotein mediated efflux by vitamin E TPGS
Collnot E, Baldes C, Wempe M, Kappl R, Hüttermann J, Hyatt J, Edgar K, Schaefer U, Lehr C (2007)
Molecular pharmaceutics, 4 (3): 465-474DOI: 10.1021/mp060121r
2004
Preparation and characterization of cationic PLGA nanospheres as DNA carriers
Ravi Kumar M, Bakowsky U, Lehr C (2004)
Biomaterials, 25 (10): 1771-1777DOI: 10.1016/j.biomaterials.2003.08.069
2001
Size-dependent bioadhesion of micro- and nanoparticulate carriers to the inflamed colonic mucosa
Lamprecht A, Schäfer U, Lehr C (2001)
Pharmaceutical research, 18 (6): 788-793DOI: 10.1023/A:1011032328064