Alison Müller studied molecular biology at the Westphalian University in Recklinghausen, where she obtained her bachelor's degree. In 2021, she switched to Saarland University and completed her master's degree in the department of human- and molecular biology. During her master's studies, she was employed as a student assistant at HIPS and worked on the isolation and characterization of myxobacteria from soil samples as part of the "Sample das Saarland" project, with the aim of identifying new bioactive microbial natural products. She did her master thesis in the Microbial Natural Products group of Prof. Dr. Rolf Müller. The subject of the thesis was the genetic modification of a biosynthetic gene cluster for heterologous expression and production of novel antibacterial darobactin analogues and their characterization.

She returned as a PhD student to HIPS in May 2023 and has since joined the Department of Microbial Natural Products. Her research is mainly focused on genetic engineering of microbial secondary metabolite biosynthetic pathways and establishment of novel heterologous expression systems in different microorganisms.