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Dr Carsten Seyfert

About

Carsten studied Molecular Biology at the University of Basel. During his Master’s studies, he worked in the group of Christoph Dehio in the Department of Infection Biology, where he established a nanobody expression platform for the characterization of Bartonella effector proteins (Beps) and contributed to elucidating the first complete structure of a Bep. After an internship at Octapharma Biopharmaceuticals (Heidelberg), he began his PhD in 2020 at HIPS in the department of Microbial Natural Products led by Rolf Müller. 

His doctoral research focused on biosynthetic gene clusters responsible for antibacterial secondary metabolites, aiming to elucidate natural product biosynthesis and enable the development of new antibiotics through heterologous expression. Using structure- and activity-guided optimization strategies, he developed and characterized novel derivatives of the Darobactin class through targeted genetic engineering and process optimization.

Currently, Carsten is a postdoctoral researcher in the department of Microbial Natural Products, where he coordinates biotechnological projects and supervises PhD candidates. Within the Darobactin team, he shares responsibility for CRO studies to further advance this antibiotic class. He also contributes to grant applications and supports the strategic development of the IP portfolio.


2024

In Vivo Activity Profiling of Biosynthetic Darobactin D22 against Critical Gram-Negative Pathogens

Kany A, Fries F, Seyfert C, Porten C, Deckarm S, Chacón Ortiz M, Dubarry N, Vaddi S, Große M, Bernecker S, …, Herrmann J, Müller R (2024)

ACS Infect. Dis. 10 (12): 4337-4346DOI: 10.1021/acsinfecdis.4c00687

Thermo-amplifier circuit in probiotic E. coli for stringently temperature-controlled release of a novel antibiotic

Dey S, Seyfert C, Fink-Straube C, Kany A, Müller R, Sankaran S (2024)

Journal of biological engineering 18 (1)DOI: 10.1186/s13036-024-00463-y

Harnessing Gram-negative bacteria for novel anti-Gram-negative antibiotics

Birkelbach J, Seyfert C, Walesch S, Müller R (2024)

Microbial Biotechnology 17 (11)DOI: 10.1111/1751-7915.70032

Investigation of the Odilorhabdin Biosynthetic Gene Cluster Using NRPS Engineering

Präve L, Seyfert C, Bozhüyük K, Racine E, Müller R, Bode H (2024)

Angewandte Chemie (International ed. in English)DOI: 10.1002/anie.202406389

2023

Darobactine: eine neue Antibiotikaklasse in Entwicklung

Seyfert C, Porten C, Müller R (2023)

Biospektrum 29 (5): 539-541DOI: 10.1007/s12268-023-1988-6

New Genetically Engineered Derivatives of Antibacterial Darobactins Underpin Their Potential for Antibiotic Development

Seyfert C, Müller A, Walsh D, Birkelbach J, Kany A, Porten C, Yuan B, Krug D, Herrmann J, Marlovits T, Hirsch A, Müller R (2023)

J. Med. Chem.DOI: 10.1021/acs.jmedchem.3c01660

New Genetically Engineered Derivatives of Antibacterial Darobac-tins Underpin their Potential for Antibiotic Development

Seyfert C, Müller A, Walsh D, Birkelbach J, Kany A, Porten C, Yuan B, Krug D, Herrmann J, Marlovits T, Hirsch A, Müller R (2023)

BookDOI: 10.26434/chemrxiv-2023-24tmj

2022

Darobactins Exhibiting Superior Antibiotic Activity by Cryo-EM Structure Guided Biosynthetic Engineering

Seyfert C, Porten C, Yuan B, Deckarm S, Panter F, Bader C, Coetzee J, Deschner F, Tehrani K, Higgins P, …, Herrmann J, Müller R (2022)

Angewandte Chemie (International ed. in English)DOI: 10.1002/anie.202214094

2021

Improved broad-spectrum antibiotics against Gram-negative pathogens via darobactin biosynthetic pathway engineering

Groß S, Panter F, Pogorevc D, Seyfert C, Deckarm S, Bader C, Herrmann J, Müller R (2021)

Chem. Sci. 12 (35): 11882-11893DOI: 10.1039/d1sc02725e