Dr Asfandyar Sikandar
About
Asfandyar Sikandar obtained his B.Sc. (Hons) in Biomolecular Science from the University of St Andrews, UK. This was followed by an M.Sc. in Biochemistry at the University of Calgary, Canada. Currently, he is pursuing his PhD under the supervision of Jun. Prof. Jesko Köhnke. His work revolves around the structural and biochemical characterization of proteins involved in natural product synthesis and bacterial defense mechanisms.
2023
Inhibitors of the Elastase LasB for the Treatment of Pseudomonas aeruginosa Lung Infections
Konstantinovic J, Kany A, Alhayek A, Abdelsamie A, Sikandar A, Voos K, Yao Y, Andreas A, Shafiei R, Loretz B, …, Haupenthal J, Hirsch A (2023)
ACS Cent. Sci. 9 (12): 2205-2215DOI: 10.1021/acscentsci.3c01102
Revision of the Absolute Configurations of Chelocardin and Amidochelocardin
Sikandar A, Popoff A, Jumde R, Mándi A, Kaur A, Elgaher W, Rosenberger L, Hüttel S, Jansen R, Hunter M, …, Kurtán T, Müller R (2023)
Angewandte Chemie (International ed. in English)DOI: 10.1002/anie.202306437
2022
Total In Vitro Biosynthesis of the Thioamitide Thioholgamide and Investigation of the Pathway
Sikandar A, Lopatniuk M, Luzhetskyy A, Müller R, Koehnke J (2022)
J. Am. Chem. Soc. 144 (11): 5136-5144DOI: 10.1021/jacs.2c00402
N-Aryl-2-iso-butylmercaptoacetamides: the discovery of highly potent and selective inhibitors of Pseudomonas aeruginosa virulence factor LasB and Clostridium histolyticum virulence factor ColH
Voos K, Yahiaoui S, Konstantinovic J, Schönauer E, Alhayek A, Sikandar A, Si Chaib K, Ramspoth T, Rox K, Haupenthal J, …, Ducho C, Hirsch A (2022)
ChemRxivDOI: 10.26434/chemrxiv-2022-fjrqr
Structure-Based Design of α-Substituted Mercaptoacetamides as Inhibitors of the Virulence Factor LasB from Pseudomonas aeruginosa
Kaya C, Walter I, Alhayek A, Shafiei R, Jézéquel G, Andreas A, Konstantinovic J, Schönauer E, Sikandar A, Haupenthal J, …, Hartmann R, Hirsch A (2022)
ACS Infect. Dis.DOI: 10.1021/acsinfecdis.1c00628
2021
Substrate-Inspired Fragment Merging and Growing Affords Efficacious LasB Inhibitors
Kaya C, Walter I, Yahiaoui S, Sikandar A, Alhayek A, Konstantinovic J, Kany A, Haupenthal J, Köhnke J, Hartmann R, Hirsch A (2021)
Angewandte Chemie (International ed. in English)DOI: 10.1002/anie.202112295
2020
The bottromycin epimerase BotH defines a group of atypical α/β-hydrolase-fold enzymes
Sikandar A, Franz L, Adam S, Santos-Aberturas J, Horbal L, Luzhetskyy A, Truman A, Kalinina O, Koehnke J (2020)
Nat Chem Biol 16 (9): 1013-1018DOI: 10.1038/s41589-020-0569-y
2019
The role of protein-protein interactions in the biosynthesis of ribosomally synthesized and post-translationally modified peptides
Sikandar A, Koehnke J (2019)
Nat. Prod. Rep. 36 (11): 1576-1588DOI: 10.1039/C8NP00064F
2018
Tackling Pseudomonas aeruginosa Virulence by a Hydroxamic Acid-Based LasB Inhibitor
Kany A, Sikandar A, Yahiaoui S, Haupenthal J, Walter I, Empting M, Köhnke J, Hartmann R (2018)
ACS chemical biology 13 (9): 2449-2455DOI: 10.1021/acschembio.8b00257
Adaptation of a Bacterial Multidrug Resistance System Revealed by the Structure and Function of AlbA
Sikandar A, Cirnski K, Testolin G, Volz C, Brönstrup M, Kalinina O, Müller R, Koehnke J (2018)
J. Am. Chem. Soc. 140 (48): 16641-16649DOI: 10.1021/jacs.8b08895
2017
Photorhabdus luminescens lectin A (PllA): A new probe for detecting α-galactoside-terminating glycoconjugates
Beshr G, Sikandar A, Jemiller E, Klymiuk N, Hauck D, Wagner S, Wolf E, Koehnke J, Titz A (2017)
J Biol Chem 292 (48): 19935-19951DOI: 10.1074/jbc.M117.812792
Thioholgamides: Thioamide-Containing Cytotoxic RiPP Natural Products
Kjaerulff L, Sikandar A, Zaburannyi N, Adam S, Herrmann J, Koehnke J, Müller R (2017)
ACS Chem. Biol. 12 (11): 2837-2841DOI: 10.1021/acschembio.7b00676
The natural product carolacton inhibits folate-dependent C1 metabolism by targeting FolD/MTHFD
Fu C, Sikandar A, Donner J, Zaburannyi N, Herrmann J, Reck M, Wagner-Döbler I, Koehnke J, Müller R (2017)
Nat Commun 8 (1)DOI: 10.1038/s41467-017-01671-5